|
| Status |
Public on Jul 01, 2022 |
| Title |
Regulation of retina microexons by srrm3 in zebrafish |
| Organism |
Danio rerio |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
To investigate the role of srrm3 as a regulator of retina microexons (RetMICs) we have sequenced adult retina from WT animals, and enucleated eyes from 5 dpf larvae WT and KO for srrm3.
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| |
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| Overall design |
24 hpf heads were collected from WT AB fish. Larvae at 48 hpf, 60 hpf and 72 hpf were collected, genotyped using fin clip, and RNA extracted from enucleated eyes from srrm3 KO and WT siblings.
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| |
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| Contributor(s) |
Ciampi M, Head S, Serrano L, Irimia M |
| Citation(s) |
35858306 |
| |
| Submission date |
Jul 25, 2021 |
| Last update date |
Aug 02, 2022 |
| Contact name |
Manuel Irimia |
| E-mail(s) |
mirimia@gmail.com
|
| Phone |
+34933160212
|
| Organization name |
Centre for Genomic Regulation
|
| Department |
Systems Biology Unit
|
| Street address |
88 Dr. Aiguader
|
| City |
Barcelona |
| State/province |
Barcelona |
| ZIP/Postal code |
08003 |
| Country |
Spain |
| |
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| Platforms (1) |
| GPL18413 |
Illumina HiSeq 2500 (Danio rerio) |
|
| Samples (11)
|
| GSM5470784 |
Eyes from 5 dpf larvae mutated for the eMIC domain of srrm3 |
| GSM5470785 |
Eyes from 5 dpf larvae WT for the eMIC domain of srrm3 |
| GSM5470786 |
Retina from WT adult fish, replicate 1 |
| GSM5470787 |
Retina from WT adult fish, replicate 2 |
| GSM6042865 |
Eyes from 48 hpf larvae mutated for the eMIC domain of srrm3. |
| GSM6042866 |
Eyes from 48 hpf larvae WT for the eMIC domain of srrm3. |
| GSM6042867 |
Eyes from 60 hpf larvae mutated for the eMIC domain of srrm3. |
| GSM6042868 |
Eyes from 60 hpf larvae WT for the eMIC domain of srrm3. |
| GSM6042869 |
Eyes from 72 hpf larvae mutated for the eMIC domain of srrm3. |
| GSM6042870 |
Eyes from 72 hpf larvae WT for the eMIC domain of srrm3. |
| GSM6042871 |
Heads from 24 hpf larvae WT for the eMIC domain of srrm3. |
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| This SubSeries is part of SuperSeries: |
| GSE180781 |
Regulation of retina microexons by srrm3 in zebrafish and by SRRM3, SRRM4 and MSI1 in human cell lines |
|
| Relations |
| BioProject |
PRJNA749559 |
| SRA |
SRP329777 |
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