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| Status |
Public on Apr 15, 2022 |
| Title |
Multiple modes of regulation control dynamic transcription patterns during the mitosis-G1 transition |
| Organism |
Homo sapiens |
| Experiment type |
Other
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| Summary |
Following cell division, genomes must reactivate gene expression patterns that reflect the identity of the cell. Here, we use PRO-seq to examine the mechanisms that reestablish transcription patterns after mitosis. We uncover regulation of the transcription cycle at multiple steps including initiation, promoter-proximal pause positioning and escape, poly-A site cleavage and termination during the mitotic-G1 transition. During mitosis, RNA polymerase activity is retained at initiation sites, albeit shifted in position relative to non-mitotic cells. This activity is strongly linked to maintenance of local chromatin architecture during mitosis and is more predictive of rapid gene reactivation than histone modifications previously associated with bookmarking. These molecular bookmarks, combined with sequence-specific transcription factors, direct expression of select cell growth and cell specific genes during mitosis followed by reactivation of functional gene groups with distinct kinetics after mitosis. This study details how dynamic regulation of transcription at multiple steps contributes to gene expression during the cell cycle.
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| Overall design |
Profiling of nascent transcription (PRO-seq) and chromatin accessibility (ATAC-seq) during and after release from mitosis in HeLa-S3 and HEK293T cells.
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| Contributor(s) |
Core L, Wojenski L |
| Citation(s) |
35357925 |
| |
| Submission date |
Jul 06, 2021 |
| Last update date |
Apr 16, 2022 |
| Contact name |
Luke A Wojenski |
| E-mail(s) |
Luke.Wojenski@UConn.edu
|
| Organization name |
University of Connecticut
|
| Department |
Molecular & Cell Biology
|
| Lab |
Core Lab
|
| Street address |
181 Auditorium Drive unit 3197 ESB, Room 306B
|
| City |
Storrs |
| State/province |
CT |
| ZIP/Postal code |
06269 |
| Country |
USA |
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| Platforms (1) |
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| Samples (20)
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| Relations |
| BioProject |
PRJNA744224 |
| SRA |
SRP327193 |
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