|
| Status |
Public on Mar 01, 2005 |
| Title |
Isolation and angiogenesis by endothelial progenitors in the fetal liver |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
While others have reported that fetal liver contains a population of endothelial progenitors based on expression of cell surface markers or culture assays, this is the first proof of a CD31+Sca1+ progenitor by demonstrating highly efficient in vivo angiogenesis and a direct connection to the host vasculature. We have developed a novel isolation method based on collagenase digestion and culture on a fetal liver-derived feeder layer and demonstrate that the feeder cells or their supernatants are required for endothelial progenitor survival and proliferation. Proteogenomic profiling of the endothelial progenitors and the feeder cells was done with tandem mass spectrometry proteomics using MudPIT and gene transcript expression profiling using high density DNA microarrays. This approach identified a number of gene transcripts, proteins and candidate growth factor pathways that are likely to be involved in endothelial progenitor growth, differentiation and angiogenesis.
Keywords = LEP - Liver Endothelial progenitor
Keywords = Feeder Cells
Keywords = Angiogenesis
Keywords = Proteogenomics
Keywords = Growth Factors
Keywords = Fetal liver
Keywords: parallel sample
|
| |
|
| Web link |
http://www.scripps.edu/mem/eht/salomon
|
| |
|
| Contributor(s) |
Cherqui S, Kurian SM, Schussler O, Hewel J, Yates JR, Salomon DR |
| Citation(s) |
16099996 |
| |
| Submission date |
Sep 03, 2004 |
| Last update date |
Jan 08, 2019 |
| Contact name |
Sunil Kurian |
| E-mail(s) |
smkurian@scripps.edu
|
| Phone |
858-784-7759
|
| Organization name |
The Scripps Research Institute
|
| Department |
Molecular and Experimental Medicine
|
| Street address |
10550 N Torrey Pines Road
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92037 |
| Country |
USA |
| |
|
| Platforms (2) |
| GPL339 |
[MOE430A] Affymetrix Mouse Expression 430A Array |
| GPL8321 |
[Mouse430A_2] Affymetrix Mouse Genome 430A 2.0 Array |
|
| Samples (7)
|
| GSM30082 |
LEP - Liver Endothelial Progenitor (Mus musculus) |
| GSM30083 |
FEEDER - Feeder Cell Layer from Fetal Liver |
| GSM30084 |
LEP Sca1+ve - Liver Endothelial Progenitor Sca1+ve cells |
| GSM30085 |
LEP Sca1-ve - Liver Endothelial Sca1-ve Cells |
| GSM101117 |
LEP Sca1+ve - Liver Endothelial Progenitor Sca1+ve cells_sample2 |
| GSM101118 |
LEP Sca1-ve - Liver Endothelial Progenitor Sca1-ve cells _sample 2 |
| GSM101119 |
LEP Sca1-ve - Liver Endothelial Progenitor Sca1-ve cells Sample 3 |
|
| Relations |
| BioProject |
PRJNA90345 |
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