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| Status |
Public on May 24, 2021 |
| Title |
Sustained viremia suppression by SHIVsf162p3cn-recalled effector-memory CD8+ T cells after PD1-based vaccination |
| Organism |
Macaca mulatta |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
HIV-1 functional cure requires sustained viral suppression without antiretroviral therapy. While effector-memory CD8+ T lymphocytes are essential for viremia control, few vaccines elicit such cellular immunity that could be potently recalled upon viral infection. Here, we investigated a program death-1 (PD1)-based vaccine by fusion of simian immunodeficiency virus capsid antigen to soluble PD1. Homologous vaccinations suppressed setpoint viremia to undetectable levels in all vaccinated macaques following high-dose intravenous challenge by the pathogenic SHIVSF162P3CN. Poly-functional effector-memory CD8+ T cells were not only induced after vaccination, but were also recalled upon viral challenge for viremia control as determined by CD8 depletion. Vaccine-induced effector memory CD8+ subsets displayed high cytotoxicity-related genes by single-cell analysis. Vaccinees with sustained viremia suppression for over two years responded to boost vaccination without viral rebound. These results demonstrated that PD1-based vaccine-induced effector-memory CD8+ T cells were recalled by AIDS virus infection, providing a potential immunotherapy for functional cure.
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| Overall design |
Single cell RNA sequencing analysis was done by using FACS purified CD8+ T cells isolated from 3 macaques (B01, B02, and B03) in Group B before immunisation, 4 weeks post, and 12 weeks post immunisation.
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| Contributor(s) |
Yue M, Wong Y, Yim L, Song Y, Chen Z |
| Citation(s) |
34125864 |
| |
| Submission date |
Apr 09, 2021 |
| Last update date |
Jul 08, 2021 |
| Contact name |
Ming Yue |
| E-mail(s) |
nervym7@connect.hku.hk
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| Organization name |
The University of Hong Kong
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| Street address |
21 Sassoon Road, Pokfulam
|
| City |
Hong Kong |
| ZIP/Postal code |
999077 |
| Country |
China |
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| Platforms (1) |
| GPL27943 |
Illumina NovaSeq 6000 (Macaca mulatta) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA720908 |
| SRA |
SRP314218 |
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