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Series GSE169464 Query DataSets for GSE169464
Status Public on Aug 01, 2022
Title Dominant role of DNA methylation over H3K9me3 in ERV silencing during embryonic endoderm development [ChIP-Suv39]
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Silencing of endogenous retroviruses (ERVs) is largely mediated by repressive chromatin modifications, such as H3K9me3 and DNA methylation. Their impact on ERV silencing differs in various cell types and, no systematic analyses on the interdependence between H3K9me3 and DNA methylation have been performed in differentiated cells. Here we show that deletion of the H3K9me3 HMTase Setdb1 in mouse embryonic endoderm results in ERV de-repression in only a subset of endoderm cells. We found that de-repression is restricted to visceral endoderm descendants and does not occur in definitive endoderm cells. Deletion of Setdb1 in visceral endoderm progenitors resulted in loss of H3K9me3 and reduced DNA methylation of IAPEz, consistent with up-regulation of this ERV family. In definitive endoderm cells, loss of Setdb1 did not affect H3K9me3 nor DNA methylation, suggesting Setdb1-independent pathways for maintaining these modifications. Importantly, Dnmt1 ko resulted in ERV de-repression in both visceral and definitive endoderm cells, while H3K9me3 was unaltered. Thus, our data suggest a dominant role of DNA methylation over H3K9me3 in ERV silencing in endoderm cells. Our findings suggest, that H3K9me3 is not sufficient for ERV silencing, but rather critical for maintaining high DNA methylation.
Overall design H3K9me3 ChIP-seq of ES/XEN/DE cells
Contributor(s) Wang Z, Fan R, Cernilogar F, Schotta G
Citation(s) 36123357
Submission date Mar 23, 2021
Last update date Oct 04, 2022
Contact name Gunnar Schotta
Phone 089218075422
Organization name LMU Munich
Department Biomedical Center
Street address Grosshaderner Strasse 9
City Planegg-Martinsried
ZIP/Postal code 82152
Country Germany
Platforms (1)
GPL18480 Illumina HiSeq 1500 (Mus musculus)
Samples (8)
GSM5203634 H3K9me3.wt26.Con.ES.r1
GSM5203635 H3K9me3.wt26.Con.ES.r2
GSM5203636 H3K9me3.Suv39.DKO.ES.r1
This SubSeries is part of SuperSeries:
GSE139128 Dominant role of DNA methylation over H3K9me3 for IAP silencing in endoderm
BioProject PRJNA716645
SRA SRP311845

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Supplementary file Size Download File type/resource
GSE169464_RAW.tar 1.1 Gb (http)(custom) TAR (of BIGWIG)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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