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Series GSE167225 Query DataSets for GSE167225
Status Public on Feb 24, 2021
Title Allergen-specific immunotherapy induces the suppressive secretoglobin 1A1 in cells of the lower airways
Organism Homo sapiens
Experiment type Expression profiling by array
Summary While several systemic immunomodulatory effects of allergen-specific immunotherapy (AIT) have been discovered, local anti-inflammatory mechanisms in the respiratory tract are largely unknown. We sought to elucidate local and epithelial mechanisms underlying allergen-specific immunotherapy in a genome-wide approach. We induced sputum in hay fever patients and healthy controls during the pollen peak season and stratified patients by effective allergen-immunotherapy or as untreated. Sputum was directly processed after induction and subjected to whole transcriptome RNA microarray analysis. Nasal secretions were analyzed for Secretoglobin1A1 (SCGB1A1) and IL-24 protein levels in an additional validation cohort at three defined time points during the three-year course of AIT. Subsequently, RNA was extracted and subjected to an array-based whole transcriptome analysis.
AIT inhibited pro-inflammatory CXCL8, IL24 and CCL26 mRNA expression, while SCGB1A1, IL7, CCL5, CCL23 and WNT5B mRNAs were induced independently of the asthma status and allergen season. In our validation cohort, local increase of SCGB1A1 occurred concomitantly with the reduction of local IL-24 in upper airways during the course of AIT. Additionally, SCGB1A1 was identified as a suppressor of epithelial gene expression.AIT induces a yet unknown local gene expression footprint in the lower airways that on one hand appears to be a result of multiple regulatory pathways and on the other hand reveals SCGB1A1 as novel anti-inflammatory mediator of long-term allergen specific therapeutic intervention in the local environment.
Overall design Induced sputa were performed in healthy controls, allergic rhinitis with and without concomittant asthma in and out of grass pollen season. Some of the patients received allergen-specific immunotherapy (AIT).
Contributor(s) Zissler UM, Jakwerth CA, Guerth F, Lewitan L, Rothkirch S, Davidovic M, Ulrich M, Oelsner M, Garn H, Schmidt-Weber CB, Chaker AM
Citation(s) 33528894
Submission date Feb 22, 2021
Last update date Feb 26, 2021
Contact name Ulrich M Zissler
Phone +49 (0)89 4140 3472
Organization name Technical University of Munich & Helmholtz Center Munich
Department Center of Allergy & Environment (ZAUM)
Lab Airway Immunology
Street address Biedersteiner Str. 29
City Munich
ZIP/Postal code 80802
Country Germany
Platforms (1)
GPL13607 Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Feature Number version)
Samples (40)
GSM5099842 LS011
GSM5099843 LS041
GSM5099844 LS058
BioProject PRJNA704278

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Supplementary file Size Download File type/resource
GSE167225_RAW.tar 126.0 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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