Expression profiling by high throughput sequencing
Summary
Dynamic cell identities underlie flexible developmental programs. The stomatal lineages in the Arabidopsis leaf epidermis feature asynchronous and indeterminate divisions that can be modulated by environmental cues. The products of these lineages, stomatal guard cells and pavement cells, regulate plant-atmosphere exchanges, and the epidermis as a whole influences overall leaf growth. How flexibility is encoded in development of the stomatal lineage, and how cell fates are coordinated in the leaf are open questions. Here, we offer single-cell transcriptomes to uncover models of cell differentiation within Arabidopsis leaf tissue.
Overall design
Included are raw and processed single-cell (sc)RNA-seq datasets that profile cells of the Arabidopsis stomatal lineage and developing leaf. The overall design and methods used are described in the associated manuscript.
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