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| Status |
Public on Feb 21, 2010 |
| Title |
Arabidopsis Argonaute4, Argonaute6 and Argonaute9 associated small RNAs |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
We sequenced the small RNA bound to AGO4, AGO6 and AGO9 and compared these sRNAs to other libraries isolated such as total sRNAs, ago4 mutant, drd3-1 mutant, an AGO4 D660A slicer mutant and AGO6 and AGO9 under the AGO4 promoter.
Keywords: Epigenetics
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| Overall design |
Illumina samples: There are 17 unique samples representing 2 biological replicates of AGO6IP, 2 biological replicates of AGO9 IP, 3 biological replicates of total floral small RNAs, 1 technical replicate of total floral small RNA.
454 samples: These samples comprise 3 biological replicates of FLAG AGO4 IP.
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| Contributor(s) |
Havecker ER, Wallbridge LM, Hardcastle TJ, Bush MS, Kelly KA, Dunn RM, Schwach F, Studholme DJ, Doonan JH, Baulcombe DC |
| Citation(s) |
20173091 |
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| Submission date |
Jun 10, 2009 |
| Last update date |
Jan 05, 2016 |
| Contact name |
Krys Kelly |
| Organization name |
University of Cambridge
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| Department |
Plant Sciences
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| Lab |
Baulcombe Group
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| Street address |
Downing Street
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| City |
Cambridge |
| ZIP/Postal code |
CB2 3EA |
| Country |
United Kingdom |
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| Platforms (2) |
| GPL9302 |
Illumina Genome Analyzer II (Arabidopsis thaliana) |
| GPL9386 |
454 GS (Arabidopsis thaliana) |
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| Samples (20)
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| GSM415786 |
Col-0 sRNAs (SL4_2) |
| GSM415787 |
antiAGO4 associated sRNAs (SL26) |
| GSM415788 |
antiAGO4 associated sRNAs (SL32) |
| GSM415789 |
FLAG AGO6 associated sRNAs (SL9) |
| GSM415790 |
FLAG AGO6 associated sRNAs (SL10) |
| GSM415791 |
antiAGO9 associated sRNAs (SL11) |
| GSM415792 |
antiAGO9 associated sRNAs (SL12) |
| GSM415793 |
AGO4 Promoter:FLAG AGO4 associated sRNAs (SL234B) |
| GSM415794 |
AGO4 Promoter:FLAG AGO6 associated sRNAs (SL234C) |
| GSM415795 |
AGO4 Promoter:FLAG AGO9 associated sRNAs (SL234F) |
| GSM415796 |
drd3-1 sRNAs (SL235B) |
| GSM415797 |
ago4-3 AGO4P:AGO4 D660A sRNAs (SL235H) |
| GSM415798 |
Col-2 sRNAs (SL24_1) |
| GSM415799 |
ago4-3 sRNAs (SL25_1) |
| GSM415800 |
Replicate 1 of FLAG AGO4 associated small RNAs immunopurified from a transgene expressed in Columbia ecotype Arabidopsis |
| GSM415801 |
Replicate 2 of FLAG AGO4 associated small RNAs immunopurified from a transgene expressed in Columbia ecotype Arabidopsis |
| GSM415802 |
Replicate 3 of FLAG AGO4 associated small RNAs immunopurified from a transgene expressed in Columbia ecotype Arabidopsis |
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| Relations |
| BioProject |
PRJNA116171 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE16545_RAW.tar |
66.8 Mb |
(http)(custom) |
TAR (of FA) |
| Processed data included within Sample table |
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