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| Status |
Public on Nov 12, 2020 |
| Title |
Anterograde Regulation of Nuclear-encoded Mitochondrial Genes and FGF21 Signaling by Hepatic Histone Demethylase LSD1 [RNA-seq] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Mitochondrial biogenesis and function are controlled by anterograde regulatory pathways involving more than one thousand nuclear-encoded proteins. Transcriptional networks controlling the nuclear-encoded mitochondrial genes remain fully elucidated. Here we show that histone demethylase LSD1 knockout from adult mouse liver (LSD1-LKO) reduces one-third of all nuclear-encoded mitochondrial genes and decreases mitochondrial biogenesis and function. ChIP-seq analysis shows that LSD1 and LSD1-targeted H3K4me2 modulate the expression of mitochondrial genes in liver.
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| Overall design |
RNA-seq analysis of gene expression in liver of LSD1-LKO and controls. To generate LSD1-LKO mice, AAV8-TBG-cre was injected through tail vein into LSD1-lox/lox mice at 6-7 weeks of age. AAV8-TBG-GFP was injected to LSD1-lox/lox mice as controls. One week after the injections, the mice were fed a high fat diet for 12 weeks.
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| Contributor(s) |
Cao Y, Sun Q, Yang Q |
| Citation missing |
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| |
| Submission date |
Nov 11, 2020 |
| Last update date |
Nov 12, 2020 |
| Contact name |
QIN YANG |
| E-mail(s) |
qin.yang@uci.edu
|
| Phone |
949-824-0232
|
| Organization name |
University of California, Irvine
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| Department |
Physiology and Biophysics
|
| Street address |
825 Health Science Rd.
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| City |
Irvine |
| ZIP/Postal code |
92697 |
| Country |
USA |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA676133 |
| SRA |
SRP292209 |
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