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| Status |
Public on May 01, 2010 |
| Title |
Identification of Genes Preferentially Required for Growth of p53-Deficient Human Cancer Cells |
| Organism |
Homo sapiens |
| Experiment type |
Other
|
| Summary |
We describe a synthetic interaction screen to identify genes preferentially required for proliferation of p53-deficient human cancer cells. An isogenic pair of p53+/+ and p53-/- human colorectal HCT116 cell lines was stably transduced with a short hairpin RNA (shRNA) library encoded by lentiviruses that integrate in the genomic DNA of the host. Comparison of the frequency of each type of integrated lentivirus in the 2 cell types at an early time point (40hrs) and after about 12 mitotic cycles (10days), reveals genes whose knockdown preferentially impaired growth of p53-/- or p53+/+ HCT116 cells.
Keywords: Functional Genomics
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| Overall design |
The region of the integrated lentiviruses is PCR amplified from genomic DNA of the two transduced cell populations at an early and a late time point.
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| Contributor(s) |
Xie L, Gazin C, Zhu LJ, Debily M, Kittler EL, Zapp ML, Lapointe D, Gobeil S, Virbasius C, Green MR |
| Citation(s) |
23284306 |
| Submission date |
May 05, 2009 |
| Last update date |
May 15, 2019 |
| Contact name |
Michael R. Green |
| E-mail(s) |
michael.green@umassmed.edu
|
| Organization name |
University of Massachusetts Medical School
|
| Department |
Molecular, Cell and Cancer Biology
|
| Lab |
Michael R. Green
|
| Street address |
364 Plantation Street
|
| City |
Worcester |
| State/province |
MA |
| ZIP/Postal code |
01605 |
| Country |
USA |
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| Platforms (1) |
| GPL9052 |
Illumina Genome Analyzer (Homo sapiens) |
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| Samples (4)
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| Relations |
| SRA |
SRP002515 |
| BioProject |
PRJNA115543 |
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