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| Status |
Public on Feb 12, 2021 |
| Title |
Targeting cholesterol metabolism as efficient antiviral strategy against the Hepatitis E virus |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
We used whole genome expression profiling to analyze the effects of Hepatitis E Virus (HEV) on the mRNA transcriptome of A549/D3 cells - particularly to identify differentially expressed genes which are associated with cholesterol metabolism.
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| Overall design |
Gene expression profiles of persistently HEV-infected A549 cells (Johne R, Reetz J, Ulrich RG, Machnowska P, Sachsenröder J, Nickel P et al. An ORF1-rearranged hepatitis E virus derived from a chronically infected patient efficiently replicates in cell culture. Journal of viral hepatitis 2014;21(6):447–56.) were analyzed after 4 and 12 days of culturing. At the same timepoints gene expression profiles of uninfected A549/D3 (Schemmerer M, Apelt S, Trojnar E, Ulrich RG, Wenzel JJ, Johne R. Enhanced Replication of Hepatitis E Virus Strain 47832c in an A549-Derived Subclonal Cell Line. Viruses 2016;8(10).) cells were analyzed. 3 biological replicates were used.
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| Contributor(s) |
Glitscher M, Heiler Martin D, Woytinek K, Schmidt B, Tabari D, Scholl C, Stingl J, Seelow E, Choi M, Hildt E |
| Citation(s) |
33601063 |
| |
| Submission date |
Sep 11, 2020 |
| Last update date |
Mar 12, 2021 |
| Contact name |
Denna Tabari |
| Organization name |
Federal Institute for Drugs and Medical Devices
|
| Street address |
Kurt-Georg-Kiesinger Allee 3
|
| City |
Bonn |
| ZIP/Postal code |
53175 |
| Country |
Germany |
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| Platforms (1) |
| GPL17077 |
Agilent-039494 SurePrint G3 Human GE v2 8x60K Microarray 039381 (Probe Name version) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA662916 |
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