GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE156618 Query DataSets for GSE156618
Status Public on May 19, 2021
Title Replication timing data for six human cell lines under aphidicolin treatment (t0) and after release (N+1)
Organism Homo sapiens
Experiment type Other
Summary DNA replication is very well orchestrated in mammalian cells due to a tight regulation of the temporal order of replication origin activation, known as the replication timing program. The replication timing of a given replication domain is very robust and well conserved in each cell type. Upon low replication stress, the slowing of replication forks induces delayed replication of some fragile regions leading to DNA damage and genetic instability. Except for these fragile regions, the direct impact of low replication stress on the replication timing in different cellular backgrounds has not been explored in detail. Here we analysed DNA replication timing across the whole genome in a panel of human cell lines in the presence of low replication stress. We demonstrate that low replication stress induced by aphidicolin has a stronger impact on the replication timing of cancer cells than non-tumour cells. Strikingly, we unveiled an enrichment of specific replication domains undergoing a switch from late to early replication in some cancer cells. We found that advances in replication timing correlate with heterochromatin regions poorly sensitive to DNA damage signalling while being subject to an increase of chromatin accessibility in response to aphidicolin. Finally, our data indicate that, following release from replication stress conditions, replication timing advances can be inherited by the next cellular generation, suggesting a new mechanism by which some cancer cells would adapt to cellular or environmental stress.
Overall design The 6 human cell lines were treated with aphidicolin 0.2µM or DMSO during adapted duration. T0 correspond to the replication timing of cells under the treatment and N+1 correspond to the replication timing of cells released from the drug during appropriate duration.
Biological replicates for each conditions (37 samples)
Contributor(s) Cadoret J
Citation(s) 34066960
Submission date Aug 21, 2020
Last update date Jul 15, 2021
Contact name Valérie Bergoglio
Phone 0582741655
Organization name INSERM UMR1037
Street address 2 avenue Hubert Curien
City Toulouse cedex 1
ZIP/Postal code 31037
Country France
Platforms (1)
GPL10123 Agilent-022060 SurePrint G3 Human CGH Microarray 4x180K (Feature Number version)
Samples (37)
GSM4734938 HCT116 replication timing in DMSO condition - Rep1
GSM4734939 HCT116 replication timing in APH condition - Rep1
GSM4734940 HCT116 replication timing in DMSO condition - Rep2
BioProject PRJNA658525

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE156618_HCT116_DMSO_log_ratio.txt.gz 4.8 Mb (ftp)(http) TXT
GSE156618_HCT116_DMSO_vs_APH_N+1.bed.gz 274 b (ftp)(http) BED
GSE156618_HCT116_DMSO_vs_APH_t0.bed.gz 1.2 Kb (ftp)(http) BED
GSE156618_HCT116_N+1_APH_log_ratio.txt.gz 4.8 Mb (ftp)(http) TXT
GSE156618_HCT116_t0_APH_log_ratio.txt.gz 4.8 Mb (ftp)(http) TXT
GSE156618_K562_DMSO_log_ratio.txt.gz 4.8 Mb (ftp)(http) TXT
GSE156618_K562_DMSO_vs_APH_N+1.bed.gz (ftp)(http) BED
GSE156618_K562_DMSO_vs_APH_t0.bed.gz 883 b (ftp)(http) BED
GSE156618_K562_N+1_APH_log_ratio.txt.gz 4.8 Mb (ftp)(http) TXT
GSE156618_K562_t0_APH_log_ratio.txt.gz 4.8 Mb (ftp)(http) TXT
GSE156618_MRC5N_DMSO_log_ratio.txt.gz 4.8 Mb (ftp)(http) TXT
GSE156618_MRC5N_DMSO_vs_APH_N+1.bed.gz 253 b (ftp)(http) BED
GSE156618_MRC5N_DMSO_vs_APH_t0.bed.gz 746 b (ftp)(http) BED
GSE156618_MRC5N_N+1_APH_log_ratio.txt.gz 3.5 Mb (ftp)(http) TXT
GSE156618_MRC5N_t0_APH_log_ratio.txt.gz 4.8 Mb (ftp)(http) TXT
GSE156618_RAW.tar 1.9 Gb (http)(custom) TAR (of TXT)
GSE156618_RKO_DMSO_vs_APH_N+1.bed.gz 401 b (ftp)(http) BED
GSE156618_RKO_DMSO_vs_APH_t0.bed.gz 788 b (ftp)(http) BED
GSE156618_RKO_N+1_APH_log_ratio.txt.gz 4.8 Mb (ftp)(http) TXT
GSE156618_RKO_N+1_DMSO_log_ratio.txt.gz 4.8 Mb (ftp)(http) TXT
GSE156618_RKO_t0_APH_log_ratio.txt.gz 4.8 Mb (ftp)(http) TXT
GSE156618_RKO_t0_DMSO_log_ratio.txt.gz 4.8 Mb (ftp)(http) TXT
GSE156618_RPE-1_DMSO_log_ratio.txt.gz 4.8 Mb (ftp)(http) TXT
GSE156618_RPE-1_DMSO_vs_APH_N+1.bed.gz 108 b (ftp)(http) BED
GSE156618_RPE-1_DMSO_vs_APH_t0.bed.gz 630 b (ftp)(http) BED
GSE156618_RPE-1_N+1_APH_log_ratio.txt.gz 4.8 Mb (ftp)(http) TXT
GSE156618_RPE-1_t0_APH_log_ratio.txt.gz 4.8 Mb (ftp)(http) TXT
GSE156618_U2OS_DMSO_log_ratio.txt.gz 4.8 Mb (ftp)(http) TXT
GSE156618_U2OS_DMSO_vs_APH_N+1.bed.gz 154 b (ftp)(http) BED
GSE156618_U2OS_DMSO_vs_APH_t0.bed.gz 964 b (ftp)(http) BED
GSE156618_U2OS_N+1_APH_log_ratio.txt.gz 4.8 Mb (ftp)(http) TXT
GSE156618_U2OS_t0_APH_log_ratio.txt.gz 4.8 Mb (ftp)(http) TXT
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap