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| Status |
Public on Aug 31, 2020 |
| Title |
Genome wide expression patterns of 19 human urinary cells as well as 6 primary urinary cells and 17 corresponding iPSCs of human, gorilla and orangutan |
| Organisms |
Gorilla gorilla; Pongo abelii; Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Comparing the molecular and cellular properties among primates is crucial to better understand human evolution and biology. However, it is difficult or ethically even impossible to collect matched tissues from many primates, especially during development. An alternative is to model different cell types and their development using induced pluripotent stem cells (iPSCs). These can be generated from many tissue sources, but non-invasive sampling would decisively broaden the spectrum of non-human primates that can be investigated. Here, we report the generation of primate iPSCs from urine samples. We first validate and optimize the procedure using human urine samples and show that Sendai virus transduction of reprogramming factors into urinary cells efficiently generates integration-free iPSCs, which maintain their pluripotency under feeder-free culture conditions. We demonstrate that this method is also applicable to gorilla and orangutan urinary cells isolated from a non-sterile zoo floor. We characterize the urinary cells, iPSCs and derived neural progenitor cells using karyotyping, immunohistochemistry, differentiation assays and RNA-sequencing. We show that the urine-derived human iPSCs are indistinguishable from well characterized PBMC-derived human iPSCs and that the gorilla and orangutan iPSCs are well comparable to the human iPSCs. In summary, this study introduces a novel and efficient approach to generate primate iPSCs non-invasively from urinary samples.
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| Overall design |
RNA-seq of 6 urinary cells and 17 iPSC lines of human, gorilla and orangutan and RNA-seq of 19 single urinary cell colonies from six human individuals
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| Contributor(s) |
Geuder J |
| Citation(s) |
33568724 |
| |
| Submission date |
Aug 07, 2020 |
| Last update date |
Feb 16, 2021 |
| Contact name |
Johanna Geuder |
| E-mail(s) |
geuder@bio.lmu.de
|
| Organization name |
LMU
|
| Street address |
Großhadernerstr. 2
|
| City |
Planegg-Martinsried |
| ZIP/Postal code |
82152 |
| Country |
Germany |
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| Platforms (3) |
| GPL18460 |
Illumina HiSeq 1500 (Homo sapiens) |
| GPL28986 |
Illumina HiSeq 1500 (Pongo abelii) |
| GPL28987 |
Illumina HiSeq 1500 (Gorilla gorilla) |
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| Samples (42)
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| Relations |
| BioProject |
PRJNA655935 |
| SRA |
SRP276690 |
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