|
| Status |
Public on Mar 04, 2021 |
| Title |
Unfolded protein response (UPR) and endoplasmic reticulum (ER) stress in acromegaly |
| Organism |
Danio rerio |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Acromegaly is a growth hormone (GH) excess pathological condition in humans and is associated with somatic disfigurement and a wide variety of systemic manifestations such as arthritis, neuropathies, carpal tunnel syndrome, reproductive disorders, metabolic disorders, and gastrointestinal complications. Studying the effect of growth hormone (GH) excess at the cellular level could help in understanding the underlying causes of acromegaly complications. In our previous publications, we have shown that excess GH increases DNA damage and impairs DNA repair pathways in somatic cells. In addition, we revealed that excessive GH reduces the number of stem cells and causes premature aging. Exaggerated growth is one of the main features of acromegaly and therefore, in this research, we have focused on protein production and biogenesis in acromegaly and whether excess GH is associated with unfolded protein response (UPR) and endoplasmic reticulum (ER) stress. Acromegaly-associated abnormal growth can be caused by hypertrophy or hyperplasia. Using pathway enrichment analysis of RNA seq data from our Zebrafish Acromegaly Model and Flow Cytometry Analysis, here we show a progressive increase in hepatocyte cell size and enrichment of hypertrophy pathways in the muscle and liver. That was associated with a progressive enrichment of protein production pathways and ribosome biogenesis. Moreover, using GSEA (Gene Set Enrichment Analysis) of acromegaly RNA seq data, here we show that endoplasmic reticulum (ER) stress is an accompanying feature of acromegaly disease. Our model exhibited endoplasmic reticulum (ER) stress in various organs, especially in the brain.
|
| |
|
| Overall design |
Total mRNA obtained from 1-years old acromegaly zebrafish model muscle, brain, and liver compared to aged zebrafish (3-years-old) and wild-type (WT) zebrafish were generated by deep sequencing using Illumina.
>>>Raw data are unavailable for this series due to file loss<<<
|
| |
|
| Contributor(s) |
Elbialy AA |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Jul 03, 2020 |
| Last update date |
Mar 05, 2021 |
| Contact name |
Shigeharu Kinoshita |
| Organization name |
The University of Tokyo
|
| Department |
Aquatic Bioscience
|
| Lab |
Aquatic Molecular Biology and Biotechnology
|
| Street address |
1-1-1,Yayoi, Bunkyo, Tokyo, 113-8657 Japan room 251
|
| City |
Tokyo |
| State/province |
Bunkyo |
| ZIP/Postal code |
113-8657 |
| Country |
Japan |
| |
|
| Platforms (1) |
| GPL18413 |
Illumina HiSeq 2500 (Danio rerio) |
|
| Samples (33)
|
|
| Relations |
| BioProject |
PRJNA643979 |
Less...
More...