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| Status |
Public on Sep 20, 2020 |
| Title |
Bisulfite (methylome) sequencing of progenies of Col-0 grafted on msh1; Col-0 grafted on dcl2,3,4,msh1; Col-0 grafted on Col-0; msh1 mutant; dcl2,3,4,msh1 quadruple mutant in Arabidopsis [msh1_graft_BS-seq] |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Methylation profiling by high throughput sequencing
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| Summary |
Col-0 floral stem was grafted on the msh1 mutant (Col-0/msh1); on the dcl2,3,4,msh1 quadruple mutant (Col-0/dcl2,3,4,msh1); on Col-0 (Col-0/Col-0). Seeds were collected from the grafted Col-0 scion after grafts were established. Seed coming from the graft then were grown on the peat mix, leaf tissue was collected at the bolting and used for the bisulfite sequencing (methylome). Tissue from the msh1 mutant and dcl2,3,4,msh1 quadruple mutants used as rootstocks was similarly collected at the bolting stage and used for the bisulfite sequencing.
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| Overall design |
3 progenies plants from each Col-0 on msh1; Col-0 on dcl2,3,4,msh1; Col-0 on Col-0 grafts were sequenced. 3 plants from msh1 mutant and dcl2,3,4,msh1 quadruple mitant rootstocks were sequenced.
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| Contributor(s) |
Kundariya H, Yang X, Mackenzie S |
| Citation(s) |
33093443 |
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| Submission date |
Jun 16, 2020 |
| Last update date |
Nov 02, 2020 |
| Contact name |
Hardik Kundariya |
| E-mail(s) |
hsk13.psu@gmail.com
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| Organization name |
The Pennsylvania State University
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| Street address |
361 N. Frear
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| City |
University Park |
| State/province |
PA |
| ZIP/Postal code |
16801 |
| Country |
USA |
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| Platforms (1) |
| GPL23157 |
HiSeq X Ten (Arabidopsis thaliana) |
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| Samples (15)
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| This SubSeries is part of SuperSeries: |
| GSE152570 |
Heritable enhanced growth vigor through grafting is associated with the RdDM pathway in plants |
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| Relations |
| BioProject |
PRJNA639731 |
| SRA |
SRP267488 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE152564_RAW.tar |
2.5 Gb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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