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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Jun 11, 2020 |
| Title |
Transcriptional changes during astrocyte maturation in vivo and in culture |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Analysis of gene expression during astrocyte differentiation and maturation in vivo and in vitro in mouse, and investigation of transcription factors and extrinsic conditions influencing maturation
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| Overall design |
In vivo astrocytes were purified from mice using anti-ACSA2 MACS at indicated ages. In vitro astrocytes were differentiated from cultured neural stem/progenitor cells (cultured in EGF/FGF2 medium) using BMP4 for 14 days (without EGF/FGF2). Where indicated, astrocytes in vitro were infected with transgenes and gene expression induced from day 7 to 14, or astrocytes differentiated for 7 days in BMP4 in normal 2D cultures or in 3D cultures were matured for 7 days in basal medium or FGF2 containing medium. Cells were analysed by bulk RNA-Seq.
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| Contributor(s) |
Guillemot F, Lattke M, Goldstone R |
| Citation(s) |
34267208 |
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| Submission date |
Jun 10, 2020 |
| Last update date |
Jul 27, 2021 |
| Contact name |
Michael Lattke |
| E-mail(s) |
michael.lattke@yahoo.de, m.lattke@imperial.ac.uk
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| Organization name |
Imperial College London
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| Department |
Brain Sciences
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| Street address |
Hammersmith Hospital Campus, Du Cane Road
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| City |
London |
| ZIP/Postal code |
W12 0NN |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (45)
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| GSM4609494 |
Astr in vitro, prep X6 [RNA-Seq] |
| GSM4609495 |
Astr in vitro, prep X8 [RNA-Seq] |
| GSM4609496 |
Astr in vitro, prep X9 [RNA-Seq] |
| GSM4609497 |
Cortical Astr, P4, prep 1 [RNA-Seq] |
| GSM4609498 |
Cortical Astr, P4, prep 2 [RNA-Seq] |
| GSM4609499 |
Cortical Astr, P4, prep 3 [RNA-Seq] |
| GSM4609500 |
Cortical Astr, 2m, prep 1 [RNA-Seq] |
| GSM4609501 |
Cortical Astr, 2m, prep 2 [RNA-Seq] |
| GSM4609502 |
Cortical Astr, 2m, prep 3 [RNA-Seq] |
| GSM4609503 |
Astr in vitro, prep X6, EGFP [RNA-Seq] |
| GSM4609504 |
Astr in vitro, prep X8, EGFP [RNA-Seq] |
| GSM4609505 |
Astr in vitro, prep X9, EGFP [RNA-Seq] |
| GSM4609506 |
Astr in vitro, prep X6, EGFP (2) [RNA-Seq] |
| GSM4609507 |
Astr in vitro, prep X8, EGFP (2) [RNA-Seq] |
| GSM4609508 |
Astr in vitro, prep X9, EGFP (2) [RNA-Seq] |
| GSM4609509 |
Astr in vitro, prep X6, Rorb [RNA-Seq] |
| GSM4609510 |
Astr in vitro, prep X8, Rorb [RNA-Seq] |
| GSM4609511 |
Astr in vitro, prep X9, Rorb [RNA-Seq] |
| GSM4609512 |
Astr in vitro, prep X6, Dbx2 [RNA-Seq] |
| GSM4609513 |
Astr in vitro, prep X8, Dbx2 [RNA-Seq] |
| GSM4609514 |
Astr in vitro, prep X9, Dbx2 [RNA-Seq] |
| GSM4609515 |
Astr in vitro, prep X6, Lhx2 [RNA-Seq] |
| GSM4609516 |
Astr in vitro, prep X8, Lhx2 [RNA-Seq] |
| GSM4609517 |
Astr in vitro, prep X9, Lhx2 [RNA-Seq] |
| GSM4609518 |
Astr in vitro, prep X6, Fezf2 [RNA-Seq] |
| GSM4609519 |
Astr in vitro, prep X8, Fezf2 [RNA-Seq] |
| GSM4609520 |
Astr in vitro, prep X9, Fezf2 [RNA-Seq] |
| GSM4609521 |
Astr in vitro, prep X6, Rorb and Fezf2 [RNA-Seq] |
| GSM4609522 |
Astr in vitro, prep X8, Rorb and Fezf2 [RNA-Seq] |
| GSM4609523 |
Astr in vitro, prep X9, Rorb and Fezf2 [RNA-Seq] |
| GSM4609524 |
Astr in vitro, prep X6, BMP 7d, basal 7d [RNA-Seq] |
| GSM4609525 |
Astr in vitro, prep X8, BMP 7d, basal 7d [RNA-Seq] |
| GSM4609526 |
Astr in vitro, prep X9, BMP 7d, basal 7d [RNA-Seq] |
| GSM4609527 |
Astr in vitro, prep X6, BMP 7d, FGF2 7d [RNA-Seq] |
| GSM4609528 |
Astr in vitro, prep X8, BMP 7d, FGF2 7d [RNA-Seq] |
| GSM4609529 |
Astr in vitro, prep X9, BMP 7d, FGF2 7d [RNA-Seq] |
| GSM4609530 |
Astr in vitro, prep X6, 3D, BMP 7d, basal 7d [RNA-Seq] |
| GSM4609531 |
Astr in vitro, prep X8, 3D, BMP 7d, basal 7d [RNA-Seq] |
| GSM4609532 |
Astr in vitro, prep X9, 3D, BMP 7d, basal 7d [RNA-Seq] |
| GSM4609533 |
Astr in vitro, prep X6, 3D, BMP 7d, FGF2 7d [RNA-Seq] |
| GSM4609534 |
Astr in vitro, prep X8, 3D, BMP 7d, FGF2 7d [RNA-Seq] |
| GSM4609535 |
Astr in vitro, prep X9, 3D, BMP 7d, FGF2 7d [RNA-Seq] |
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| This SubSeries is part of SuperSeries: |
| GSE152223 |
Extensive transcriptional and chromatin changes underlie astrocyte maturation in vivo and in culture |
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| Relations |
| BioProject |
PRJNA638661 |
| SRA |
SRP266817 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE152221_RNA_deseq_results_Rorb_Fezf2_combined_OE.csv.gz |
15.8 Mb |
(ftp)(http) |
CSV |
| GSE152221_RNA_deseq_results_TF_OE.csv.gz |
25.9 Mb |
(ftp)(http) |
CSV |
| GSE152221_RNA_deseq_results_in_vivo_vs_in_vivo_baseline.csv.gz |
11.7 Mb |
(ftp)(http) |
CSV |
| GSE152221_RNA_deseq_results_maturation_conditions.csv.gz |
13.7 Mb |
(ftp)(http) |
CSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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