![](/coreweb/template1/pix/main_left_bg.gif) |
![](/coreweb/template1/pix/pixel.gif) |
GEO help: Mouse over screen elements for information. |
|
Status |
Public on Oct 19, 2009 |
Title |
Transcriptomic response of young adult N2, xpa1, and glp1 nematodes 3h after exposure to 50 J/m2 UVC radiation at 25°C |
Organism |
Caenorhabditis elegans |
Experiment type |
Expression profiling by array
|
Summary |
We compared the transcriptomic response to UVC radiation in widltype (N2), DNA repair-deficient (xpa-1), and germ cell-deficient (glp-1) young adult nematodes. We were interested in the wildtype response to this stressor, and postulated that the early (3h post-exposure) difference might be different in nucleotide excision repair-deficient (xpa-1) nematodes. We also hypothesized that the response would be different in young adults composed entirely of somatic cells (glp-1 strain at this temperature), compared to germ cell-bearing and gravid young adults (N2 and xpa-1). Finally, we compared constitutive differences in gene expression between the strains.
|
|
|
Overall design |
Young adult (24 h post-vulval crescent L4 stage) N2, xpa-1, and glp-1 nematodes were reared at 25°C and exposed to 50 J/m2 UVC radiation on K-agar plates without food as described (Meyer et al., 2007 Genome Biology 8(5):R70), placed on OP50-seeded plates for 2 h 45 minutes, washed off plates and rinsed with K-medium 3 times (~5 minutes each) by allowing settling, resuspending, settling again, etc. Nematodes were frozen and RNA extracted. The experiment was performed twice, once in 2007 and once in 2008, with 2 replicates each time, for a total “n” of 4 per strain per treatment (control vs UVC-exposed).
|
|
|
Contributor(s) |
Meyer JN, Andrews DM, Lehmann DW, Van Houten B |
Citation(s) |
19879883 |
|
Submission date |
Mar 09, 2009 |
Last update date |
Sep 25, 2019 |
Contact name |
NIEHS Microarray Core |
E-mail(s) |
microarray@niehs.nih.gov, liuliw@niehs.nih.gov
|
Organization name |
NIEHS
|
Department |
DIR
|
Lab |
Microarray Core
|
Street address |
111 T.W. Alexander Drive
|
City |
RTP |
State/province |
NC |
ZIP/Postal code |
27709 |
Country |
USA |
|
|
Platforms (1) |
GPL200 |
[Celegans] Affymetrix C. elegans Genome Array |
|
Samples (24)
|
|
Relations |
BioProject |
PRJNA114931 |
Supplementary file |
Size |
Download |
File type/resource |
GSE15159_RAW.tar |
51.3 Mb |
(http)(custom) |
TAR (of CEL) |
Processed data included within Sample table |
|
|
|
|
![](/coreweb/template1/pix/main_right_bg.gif) |