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| Status |
Public on Oct 19, 2009 |
| Title |
Transcriptomic response of young adult N2, xpa1, and glp1 nematodes 3h after exposure to 50 J/m2 UVC radiation at 25°C |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Expression profiling by array
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| Summary |
We compared the transcriptomic response to UVC radiation in widltype (N2), DNA repair-deficient (xpa-1), and germ cell-deficient (glp-1) young adult nematodes. We were interested in the wildtype response to this stressor, and postulated that the early (3h post-exposure) difference might be different in nucleotide excision repair-deficient (xpa-1) nematodes. We also hypothesized that the response would be different in young adults composed entirely of somatic cells (glp-1 strain at this temperature), compared to germ cell-bearing and gravid young adults (N2 and xpa-1). Finally, we compared constitutive differences in gene expression between the strains.
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| Overall design |
Young adult (24 h post-vulval crescent L4 stage) N2, xpa-1, and glp-1 nematodes were reared at 25°C and exposed to 50 J/m2 UVC radiation on K-agar plates without food as described (Meyer et al., 2007 Genome Biology 8(5):R70), placed on OP50-seeded plates for 2 h 45 minutes, washed off plates and rinsed with K-medium 3 times (~5 minutes each) by allowing settling, resuspending, settling again, etc. Nematodes were frozen and RNA extracted. The experiment was performed twice, once in 2007 and once in 2008, with 2 replicates each time, for a total “n” of 4 per strain per treatment (control vs UVC-exposed).
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| Contributor(s) |
Meyer JN, Andrews DM, Lehmann DW, Van Houten B |
| Citation(s) |
19879883 |
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| Submission date |
Mar 09, 2009 |
| Last update date |
Sep 25, 2019 |
| Contact name |
NIEHS Microarray Core |
| E-mail(s) |
microarray@niehs.nih.gov, liuliw@niehs.nih.gov
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| Organization name |
NIEHS
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| Department |
DIR
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| Lab |
Microarray Core
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| Street address |
111 T.W. Alexander Drive
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| City |
RTP |
| State/province |
NC |
| ZIP/Postal code |
27709 |
| Country |
USA |
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| Platforms (1) |
| GPL200 |
[Celegans] Affymetrix C. elegans Genome Array |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA114931 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE15159_RAW.tar |
51.3 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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