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Series GSE150521 Query DataSets for GSE150521
Status Public on Nov 04, 2020
Title β-catenin drives distinct transcriptional networks in proliferative and non-proliferative cardiomyocytes
Organisms Homo sapiens; Mus musculus
Experiment type Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Summary The inability of the adult mammalian heart to regenerate represents a fundamental barrier in heart failure management. In contrast, the neonatal heart retains a transient regenerative capacity, but the underlying mechanisms are not fully understood. Wnt/β-catenin signaling has been suggested as a key cardio-regenerative pathway. Here, we show that Wnt/β-catenin signaling potentiates neonatal mouse cardiomyocyte proliferation in vivo and immature human pluripotent stem cell-derived cardiomyocyte (hPSC-CM) proliferation in vitro. In contrast, Wnt/β-catenin signaling in adult mice is cardioprotective but fails to induce cardiomyocyte proliferation. Transcriptional profiling of neonatal mouse and hPSC-CM revealed a core Wnt/β-catenin-dependent transcriptional network governing cardiomyocyte proliferation. In contrast, β-catenin failed to re-engage this proliferative gene network in the adult heart, which instead reverted to a neonatal-like glycolytic program. These findings suggest that Wnt/β-catenin drives distinct transcriptional networks in regenerative and non-regenerative cardiomyocytes, which may contribute towards the inability of the adult heart to regenerate following injury.
Overall design 2D cardiac cells were treated with either 0.05% DMSO or 5 µM CHIR99021 for 24 hours and then sorted by FACS into cardiomyocyte (CD90-) and non-cardiomyocyte (CD90+) cell populations. Isolated cardiomycoytes from myocardial infarcted mice with AAV6 intramyocardial injections
2D cardiac cells were treated for 24 hours with 5 µm CHIR. hPSC-CMs were fixed for 10 minutes at room temperature with 1% paraformaldehyde (PFA). Cross-linking was stopped by addition 0.125M glycine for 5 minutes and ChIP-seq was performed
Contributor(s) Quaife-Ryan GA, Porrello ER, Hudson JE
Citation(s) 33144401
Submission date May 13, 2020
Last update date Nov 04, 2020
Contact name James Hudson
Organization name QIMR Berghofer Medical Research Institute
Lab Cardiac Bioengineering Laboratory
Street address 300 Herston Rd
City Brisbane
State/province QLD
ZIP/Postal code 4006
Country Australia
Platforms (3)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
GPL18460 Illumina HiSeq 1500 (Homo sapiens)
GPL21103 Illumina HiSeq 4000 (Mus musculus)
Samples (28)
GSM4551641 2D_CD90+_DMSO_1
GSM4551642 2D_CM_DMSO_1
GSM4551643 2D_CD90+_CHIR_1
BioProject PRJNA632627
SRA SRP261505

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE150521_20200506_CHIRvsDMSO_CD90+.txt.gz 1.2 Mb (ftp)(http) TXT
GSE150521_20200506_CHIRvsDMSO_CPM.txt.gz 1.5 Mb (ftp)(http) TXT
GSE150521_20200506_CHIRvsDMSO_Count_matrix.txt.gz 545.5 Kb (ftp)(http) TXT
GSE150521_20200506_CHIRvsDMSO_Myo.txt.gz 1.2 Mb (ftp)(http) TXT
GSE150521_20200506_MIP56.BCATvsGFP.txt.gz 1.1 Mb (ftp)(http) TXT
GSE150521_20200506_MIP56.BCATvsGFP_CPM.txt.gz 1.2 Mb (ftp)(http) TXT
GSE150521_20200506_MIP56.BCATvsGFP_Countmatrix.txt.gz 391.8 Kb (ftp)(http) TXT
GSE150521_H3K27ac_consensus.bed.txt.gz 522.0 Kb (ftp)(http) TXT
GSE150521_H3K4me3_consensus.bed.txt.gz 289.4 Kb (ftp)(http) TXT
GSE150521_TCF7L2_consensus.bed.txt.gz 114.9 Kb (ftp)(http) TXT
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Processed data are available on Series record

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