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| Status |
Public on Mar 27, 2009 |
| Title |
Chromatin immunoprecipitation of C/EBP delta in mouse bone-marrow macrophages stimulated with LPS for 6hr |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by genome tiling array
|
| Summary |
The innate immune system is a two-edged sword; it is absolutely required for host defense against infection, but if left uncontrolled can trigger a plethora of inflammatory diseases. Here we used systems biology approaches to predict and validate a gene regulatory network involving a dynamic interplay between the transcription factors NF-kB, C/EBPd, and ATF3 that controls inflammatory responses. We mathematically modeled transcriptional regulation of Il6 and Cebpd genes and experimentally validated the prediction that the combination of an initiator (NF-kB), an amplifier (C/EBPd) and an attenuator (ATF3) forms a regulatory circuit that discriminates between transient and persistent Toll-like receptor 4-induced signals. Our results suggest a mechanism that enables the innate immune system to detect the duration of infection and to respond appropriately.
Keywords: ChIP-chip
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| Overall design |
Two sample types with 3 biological replicates each.
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| Contributor(s) |
Litvak V, Ramsey SA, Rust AG, Diercks A, Zak DE, Kennedy KA, Lampano AE, Nykter M, Shmulevich I, Aderem A |
| Citation(s) |
19270711 |
| |
| Submission date |
Feb 12, 2009 |
| Last update date |
Oct 14, 2014 |
| Contact name |
Bruz Marzolf |
| E-mail(s) |
bmarzolf@systemsbiology.org
|
| Phone |
206-732-1352
|
| Organization name |
Institute for Systems Biology
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| Street address |
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98103 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL5811 |
[Mm_PromPR] Affymetrix Mouse Promoter 1.0R Array |
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| Samples (6)
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| GSM370899 |
0_NoStim_C-EBPdelta_BMM_G2_T1_20071105 |
| GSM370900 |
0_NoStim_C-EBPdelta_BMM_A_T1_20070315 |
| GSM370901 |
0_NoStim_C-EBPdelta_BMM_C_T1_20070315 |
|
| Relations |
| BioProject |
PRJNA112047 |
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