GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE147787 Query DataSets for GSE147787
Status Public on Dec 21, 2021
Title BCL11A promotes myeloid leukemogenesis by abrogating the transcriptional activity of PU.1 [ChIP-seq]
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary The transcriptional repressor Bcl11a is involved in hematological malignancies, B-cell development and fetal to adult hemoglobin switching, however, the molecular mechanism how Bcl11a promotes development of myeloid leukemia remains largely unknown. We found that Bcl11a cooperates with the pseudokinase Trib1 in AML development. Bcl11a promotes proliferation of Trib1-expressing AML cells both in vitro and in vivo. ChIP-seq analysis showed that Bcl11a is significantly associated on DNA-binding with PU.1 that promotes myeloid differentiation, and Bcl11a represses a number of PU.1 target genes such as Clec5a, Fcgr3 and Csf1r. The repression of PU.1 target genes by Bcl11a is achieved by both sequence-specific DNA binding activity and recruitment of corepressors by Bcl11a, and suppression of corepressor components HDAC1 and LSD1 cancelled the repressive activity. Association of high level of expression of BCL11A and worse prognosis is observed in human AML patients, and blocking of BCL11A expression upregulates PU.1 target expression, inhibits engraftment to bone marrow, and enhances myeloid differentiation of HL-60 cells, suggesting that BCL11A is involved in human myeloid malignancies via the PU.1 transcriptional activity.
Overall design Examination of binding sites of 2 transcription factors and Histone H3K27ac in a single cell line
Contributor(s) Sunami Y, Nakamura T
Citation(s) 34714913
Submission date Mar 30, 2020
Last update date Mar 22, 2022
Contact name Takuro Nakamura
Phone 81-3-3570-0462
Organization name Japanese Foundation for Cancer Research
Department The Cancer Institute
Lab Carcinogenesis
Street address 3-8-31 Ariake, Koto-ku
City Tokyo
ZIP/Postal code 135-8550
Country Japan
Platforms (1)
GPL16417 Illumina MiSeq (Mus musculus)
Samples (4)
GSM4445940 Bcl11a
GSM4445941 PU.1
GSM4445942 H3K27ac
This SubSeries is part of SuperSeries:
GSE147798 BCL11A promotes myeloid leukemogenesis by abrogating the transcriptional activity of PU.1
BioProject PRJNA616362
SRA SRP254662

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE147787_RAW.tar 565.3 Mb (http)(custom) TAR (of TDF)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap