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Series GSE143821 Query DataSets for GSE143821
Status Public on Dec 16, 2021
Title Dynamic FMR1 granule phase switch instructed by m6A modification contributes to maternal RNA decay
Organism Drosophila melanogaster
Experiment type Expression profiling by high throughput sequencing
Other
Summary Maternal RNA degradation is critical for embryogenesis and is tightly controlled by maternal RNA-binding proteins. Fragile X mental-retardation protein (FMR1) binds target mRNAs to form ribonucleoprotein (RNP) complexes/granules that control various biological processes, including early embryogenesis. However, how FMR1 recognizes target mRNAs and how FMR1-RNP granule assembly/disassembly regulates FMR1-associated mRNAs remain elusive. Here we show that Drosophila FMR1 preferentially binds mRNAs containing m6A-marked “AGACU” motif with high affinity to contributes to maternal RNA degradation. The high-affinity binding largely depends on a hydrophobic network within FMR1 KH2 domain. Importantly, this binding greatly induces FMR1 granule condensation to efficiently recruit unmodified mRNAs. The degradation of maternal mRNAs then causes granule de-condensation, allowing normal embryogenesis. Our findings reveal that sequence-specific mRNAs instruct FMR1-RNP granules to undergo a dynamic phase-switch, thus contributes to maternal mRNA decay. This mechanism may represent a general principle that regulated RNP-granules control RNA processing and normal development.
 
Overall design RNA-seq of embryos at the indicated stages were generated by deep sequencing, in duplicate, using NovaSeq platform. MeRIP-Seq of embryos at the indicated stages were generated by deep sequencing, in duplicate, using illumina Hiseq instrument.
 
Contributor(s) Zhang G, Xu Y, Wang X, Zhu Y, Wang L, Zhang W, Wang Y, Gao Y, Wu X, Cheng Y, Sun Q, Chen D
Citation(s) 35165263
Submission date Jan 16, 2020
Last update date Mar 09, 2022
Contact name Guoqiang Zhang
E-mail(s) gqzhang@ynu.edu.cn
Organization name INSTITUTE OF ZOOLOGY, CHINESE ACADEMY OF SCIENCES
Street address 1 Beichen West Road, Chaoyang District
City Beijing
ZIP/Postal code 100101
Country China
 
Platforms (2)
GPL21306 Illumina HiSeq 4000 (Drosophila melanogaster)
GPL25244 Illumina NovaSeq 6000 (Drosophila melanogaster)
Samples (48)
GSM4275002 0-0.5h M-,Z+ mettl3 and mettl14 double mutant embryos RNA-seq replicate 1
GSM4275003 0-0.5h M-,Z+ mettl3 and mettl14 double mutant embryos RNA-seq replicate 2
GSM4275004 0-0.5h wild type embryos RNA-seq replicate 1
Relations
BioProject PRJNA601768
SRA SRP242304

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE143821_MeRIP-seq_processed_data.tar.gz 87.9 Kb (ftp)(http) TAR
GSE143821_RIP-seq_processed_data.tar.gz 178.0 Kb (ftp)(http) TAR
GSE143821_RNA-seq_processed_data.tar.gz 1.9 Mb (ftp)(http) TAR
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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