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| Status |
Public on Mar 21, 2020 |
| Title |
Effects of treatment with bovine oviductal extracellular vesicles on the transcriptome of bovine day 7 in vitro produced embryos |
| Organism |
Bos taurus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Oviductal extracellular vesicles (oEVs) are emerging as key players in gamete/embryo-oviduct interactions contributing to successful pregnancy. Various positive effects of oEVs on gametes and early embryos have been found in vitro. To find out if these effects are associated with changes of embryonic gene expression, the embryonic transcriptome was analyzed after supplementation of bovine embryos with bovine oEVs during in vitro culture (IVC) in comparison to corresponding controls without oEVs. Embryos were cultured during 7 days of IVC with fresh oEVs, oEVs frozen after isolation from oviductal fluid or without oEVs (control), since a previous study showed different effects of fresh and frozen oEVs. Five pools of 10-14 embryos were analyzed for each group by low-input Illumina RNA-sequencing. Analysis of differential gene expression revealed 221 differentially expressed genes (DEGs) for the comparison of frozen oEVs vs control, 67 DEGs for fresh vs frozen oEVs, and only minor differences for the comparison fresh oEVs vs control (28 DEGs). An integrative analysis with the previously studied mRNA and small ncRNA content of bovine oEVs suggested direct effects of oEVs content on the embryonic transcriptome for the supplementation with frozen oEVs, i.e., increase of transcripts found in higher concentrations in oEVs and decrease of transcripts which are potential targets of miRNAs found in oEVs.
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| |
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| Overall design |
Supplementation of bovine embryos during in vitro culture with fresh or frozen bovine oviductal EVs and untreated controls, low-input total RNA-seq of 5 replicates (pools of 8-14 embryos) per group using Illumina HiSeq 2500
|
| Web link |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7072903/
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| |
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| Contributor(s) |
Almiñana Brines C, Bauersachs S |
| Citation(s) |
32075098 |
| |
| Submission date |
Jan 13, 2020 |
| Last update date |
Mar 22, 2020 |
| Contact name |
Stefan Michael Bauersachs |
| E-mail(s) |
stefan.bauersachs@uzh.ch
|
| Organization name |
University of Zurich
|
| Department |
Department for Farm Animals
|
| Lab |
Genetics and Functional Genomics
|
| Street address |
Eschikon 27 EHB 23.1
|
| City |
Lindau |
| State/province |
Zurich |
| ZIP/Postal code |
8315 |
| Country |
Switzerland |
| |
|
| Platforms (1) |
| GPL19172 |
Illumina HiSeq 2500 (Bos taurus) |
|
| Samples (15)
|
| GSM4263619 |
control embryos replicate 4 |
| GSM4263620 |
control embryos replicate 5 |
| GSM4263621 |
embryos supplemented with fresh oEVs replicate 1 |
| GSM4263622 |
embryos supplemented with fresh oEVs replicate 2 |
| GSM4263623 |
embryos supplemented with fresh oEVs replicate 3 |
| GSM4263624 |
embryos supplemented with fresh oEVs replicate 4 |
| GSM4263625 |
embryos supplemented with fresh oEVs replicate 5 |
| GSM4263626 |
embryos supplemented with frozen oEVs replicate 1 |
| GSM4263627 |
embryos supplemented with frozen oEVs replicate 2 |
| GSM4263628 |
embryos supplemented with frozen oEVs replicate 3 |
| GSM4263629 |
embryos supplemented with frozen oEVs replicate 4 |
| GSM4263630 |
embryos supplemented with frozen oEVs replicate 5 |
|
| Relations |
| BioProject |
PRJNA600994 |
| SRA |
SRP241824 |
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