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| Status |
Public on Dec 21, 2019 |
| Title |
Single cell RNA sequencing analysis of the mouse colonic mesenchyme (Drop-seq) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Single cell RNA sequencing of the mouse colonic mesenchyme. Mesenchymal/lamina propria cells were isolated from the middle and distal colon of wild type mice in two biological replicates. For each biological replicate the colons of n = 2 mice were pooled. The vast majority of intestinal epithelial cells were depleted by Ethylenediaminetetraacetic acid (EDTA) treatment of the tissue samples and mesenchymal/lamina propria cells were isolated after enzymatic treatment with collagenase XI and dispase. Single cell RNA sequencing was performed with the Drop-seq protocol. N =5 Drop-seq collections (samples) were processed in total, two from the first biological replicate and three from the second.
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| |
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| Overall design |
Two biological replicates (C1 and C2): one with two Drop-seq collections (samples C1A, C1B) and one with three Drop-seq collections (samples C2A, C2B, C2C)
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| |
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| Contributor(s) |
Flavell RA, Roulis M |
| Citation(s) |
32322056 |
| |
| Submission date |
Dec 20, 2019 |
| Last update date |
Apr 29, 2020 |
| Contact name |
Richard Flavell |
| E-mail(s) |
richard.flavell@yale.edu
|
| Organization name |
Yale University
|
| Department |
Department of Immunobiology
|
| Street address |
300 Cedar Street, TAC S-569
|
| City |
New Haven |
| State/province |
CT |
| ZIP/Postal code |
06511 |
| Country |
USA |
| |
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| Platforms (1) |
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| Samples (5)
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| This SubSeries is part of SuperSeries: |
| GSE142431 |
Paracrine orchestration of intestinal tumorigenesis by a confined mesenchymal niche |
|
| Relations |
| BioProject |
PRJNA597002 |
| SRA |
SRP238352 |
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