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| Status |
Public on Dec 21, 2019 |
| Title |
Single cell RNA sequencing of cells isolated from the intestinal crypts of mice bearing epithelial cell-specific Ptger4 ablation (Drop-seq) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Crypts isolated from the small intestine of mice bearing epithelial cell-specific ablation of the Ptger4 gene (VillinCrePtger4flox, "V") and normal littermate control mice (Ptger4flox, "F") were dissociated into single cell suspensions and subjected to the Drop-seq protocol. N = 2 mice per genotype were independently processed as biological replicates (V1, V2 and F1, F2). A total of three Drop-seq collections (samples) were processed for each genotype, two from the first biological replicate (V1A, V1B, F1A, F1B) and one from the second biological replicate (V2, F2).
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| |
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| Overall design |
A total of three Drop-seq collections (samples) were analyzed per genotype from two biological replicates per genotype
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| |
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| Contributor(s) |
Flavell RA, Roulis M |
| Citation(s) |
32322056 |
| |
| Submission date |
Dec 20, 2019 |
| Last update date |
Apr 29, 2020 |
| Contact name |
Richard Flavell |
| E-mail(s) |
richard.flavell@yale.edu
|
| Organization name |
Yale University
|
| Department |
Department of Immunobiology
|
| Street address |
300 Cedar Street, TAC S-569
|
| City |
New Haven |
| State/province |
CT |
| ZIP/Postal code |
06511 |
| Country |
USA |
| |
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| Platforms (1) |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE142431 |
Paracrine orchestration of intestinal tumorigenesis by a confined mesenchymal niche |
|
| Relations |
| BioProject |
PRJNA596993 |
| SRA |
SRP238348 |
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