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Series GSE142418 Query DataSets for GSE142418
Status Public on Dec 21, 2019
Title Single cell RNA sequencing of cells isolated from the intestinal crypts of mice bearing epithelial cell-specific Ptger4 ablation (Drop-seq)
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Crypts isolated from the small intestine of mice bearing epithelial cell-specific ablation of the Ptger4 gene (VillinCrePtger4flox, "V") and normal littermate control mice (Ptger4flox, "F") were dissociated into single cell suspensions and subjected to the Drop-seq protocol. N = 2 mice per genotype were independently processed as biological replicates (V1, V2 and F1, F2). A total of three Drop-seq collections (samples) were processed for each genotype, two from the first biological replicate (V1A, V1B, F1A, F1B) and one from the second biological replicate (V2, F2).
Overall design A total of three Drop-seq collections (samples) were analyzed per genotype from two biological replicates per genotype
Contributor(s) Flavell RA, Roulis M
Citation(s) 32322056
Submission date Dec 20, 2019
Last update date Apr 29, 2020
Contact name Richard Flavell
Organization name Yale University
Department Department of Immunobiology
Street address 300 Cedar Street, TAC S-569
City New Haven
State/province CT
ZIP/Postal code 06511
Country USA
Platforms (1)
GPL21273 HiSeq X Ten (Mus musculus)
Samples (6)
GSM4227181 Ptger4 epithelial knock-out-1 (V1A)
GSM4227182 Ptger4 epithelial knock-out-2 (V1B)
GSM4227183 Ptger4 flox-1 (F1A)
This SubSeries is part of SuperSeries:
GSE142431 Paracrine orchestration of intestinal tumorigenesis by a confined mesenchymal niche
BioProject PRJNA596993
SRA SRP238348

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE142418_RAW.tar 9.8 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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