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| Status |
Public on May 26, 2020 |
| Title |
Epstein-Barr virus positive gastric cancer involves enhancer activation through ATF3 |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
ATF3 binding sites on the genome and histone modification arround the ATF3 binding sites were analized by ChIP-seq. Regulation of gene expression by ATF3 on active enhacer regions were analyzed by knocked down of ATF3.
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| Overall design |
Human gastric cancer cell lines MKN7 (MKN7_WT), Epstein-barr virus infected MKN7 (MKN7_EB), NCC24 and SNU719 were transfected ATF3 targeted or non-targeted shRNA lentivirus (shATF3 or shNON respectively) followed by drug selection with puromycin for 2 days. Total RNA was extracted after the drug selection for RNA-seq. MKN7_WT cells were treated with 30 micro mol/L of CDDP for 2 days to induce ATF3 followed by ChIP.
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| Contributor(s) |
Asakawa Y, Fukuyo M, Okabe A, Kaneda A |
| Citation(s) |
32119176 |
| |
| Submission date |
Dec 03, 2019 |
| Last update date |
May 26, 2020 |
| Contact name |
Masaki Fukuyo |
| E-mail(s) |
fukuyo@chiba-u.jp
|
| Organization name |
Chiba University
|
| Department |
Department of Molecular Oncology
|
| Street address |
1-8-1 Inohana, Chuo-ku
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| City |
Chiba |
| ZIP/Postal code |
260-8670 |
| Country |
Japan |
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| Platforms (2) |
| GPL18460 |
Illumina HiSeq 1500 (Homo sapiens) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA593250 |
| SRA |
SRP234565 |
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