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Series GSE140962 Query DataSets for GSE140962
Status Public on Jan 01, 2021
Title Correlating functional heterogeneity of human dermal fibroblasts with differences in gene expression
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Recent work has identified markers of fibroblast heterogeneity in human dermis. Transforming growth factor-β1 (TGF-β1) promotes fibroblast-to-myofibroblast differentiation, characterised by the expression of α-smooth muscle actin (α-SMA). Human dermal fibroblasts (hDF), treated with TGF-β1, were assayed for differentiation, proliferation and cell shape using the Operetta imaging system. One donor hDF, derived from female 64-year-old breast skin, expressed decreased levels of α-SMA protein. The gene expression profile of this donor hDF was determined using the Agilent microarray system. Four gene candidates (Asporin, ASPN; C-X-C motif chemokine ligand 1, CXCL1; Insulin-like growth factor 1, IGF1; and Wnt family member 4, WNT4) were chosen based on expression values and validated by TaqMan qPCR. Successful knockdown of IGF1 and WNT4 was achieved using MISSION shRNA-based lentiviral treatment. Fibroblast IGF1 knockdown (shIGF1) increased α-SMA mRNA and protein expression; no effect was seen with fibroblast WNT4 knockdown (shWNT4). Here I have characterised hDF phenotype and gene expression with regard to population heterogeneity. This work highlights the role of IGF-1 signalling on α-SMA expression and dermal fibroblast fate. Targeting IGF-1 signalling could provide therapeutic benefit for skin disorders involving aberrant wound healing and excessive fibrosis.
Overall design Enzyme-derived, human dermal fibroblasts from four adult donors. Treated with/without TGF-β1 for 12h/24h and used for Agilent one colour microarray.
Contributor(s) Culley OJ, Watt FM
Citation(s) 33889572
Submission date Nov 25, 2019
Last update date Apr 27, 2021
Contact name Oliver J Culley
Phone +447500 939574
Organization name King’s College London
Department Centre for Stem Cells and Regenerative Medicine
Lab Fiona M Watt
Street address Floor 28, Tower Wing, Guy’s Hospital, Great Maze Pond
City London
ZIP/Postal code SE1 9RT
Country United Kingdom
Platforms (1)
GPL17077 Agilent-039494 SurePrint G3 Human GE v2 8x60K Microarray 039381 (Probe Name version)
Samples (48)
GSM4191458 hDF_F22Br_Control_12h_rep1
GSM4191459 hDF_M50F_TGFb1_12h_rep1
GSM4191460 hDF_F64Br_Control_12h_rep1
BioProject PRJNA591561

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Supplementary file Size Download File type/resource
GSE140962_RAW.tar 148.9 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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