|
| Status |
Public on Apr 24, 2020 |
| Title |
Ig repertoire of IgG+ murine memory B cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
BCR repertoire sequencing of isotype-switched memory B cells Aim of the study was to explore the differences of Ig repertoires of isotype-switched memory B cells between spleen and bone marrow compartments.
|
| |
|
| Overall design |
Cells from BM (tibiae, femurae and pelvic bones) and spleen of three times NP-CGG immunized-mice were isolated. Sorted cells of Mouse 1 and 2 were split into two equal aliquots (samples A, B) for BM and spleen samples as cellular (biological) replicates. Sorted cells of Mouse 3 were split into 4 samples (A, B, C, D). Biological replicates were processed independently from this point on. Isolated RNA was split and library preparation was performed in technical duplicates.
|
| |
|
| Contributor(s) |
Riedel R, Addo RK, Heinz GA, Kummer J, Heinrich F, Durek P, Mashreghi M, Radbruch A |
| Citation(s) |
32444631 |
| |
| Submission date |
Nov 01, 2019 |
| Last update date |
May 28, 2020 |
| Contact name |
Pawel Durek |
| E-mail(s) |
pawel.durek@drfz.de
|
| Organization name |
Deutsches Rheuma-Forschungszentrum
|
| Street address |
Charitéplatz 1
|
| City |
Berlin |
| ZIP/Postal code |
10117 |
| Country |
Germany |
| |
|
| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
|
| Samples (32)
|
|
| This SubSeries is part of SuperSeries: |
| GSE140133 |
Single-Cell transcriptomes and BCR sequences of IgG+ murine memory B cells from Bone Marrow and Spleen |
|
| Relations |
| BioProject |
PRJNA587123 |
| SRA |
SRP227970 |
Less...
More...