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| Status |
Public on Aug 01, 2022 |
| Title |
Dominant role of DNA methylation over H3K9me3 in ERV silencing during embryonic endoderm development [RNA-Seq] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Silencing of endogenous retroviruses (ERVs) is largely mediated by repressive chromatin modifications, such as H3K9me3 and DNA methylation. Their impact on ERV silencing differs in various cell types and, no systematic analyses on the interdependence between H3K9me3 and DNA methylation have been performed in differentiated cells. Here we show that deletion of the H3K9me3 HMTase Setdb1 in mouse embryonic endoderm results in ERV de-repression in only a subset of endoderm cells. We found that de-repression is restricted to visceral endoderm descendants and does not occur in definitive endoderm cells. Deletion of Setdb1 in visceral endoderm progenitors resulted in loss of H3K9me3 and reduced DNA methylation of IAPEz, consistent with up-regulation of this ERV family. In definitive endoderm cells, loss of Setdb1 did not affect H3K9me3 nor DNA methylation, suggesting Setdb1-independent pathways for maintaining these modifications. Importantly, Dnmt1 ko resulted in ERV de-repression in both visceral and definitive endoderm cells, while H3K9me3 was unaltered. Thus, our data suggest a dominant role of DNA methylation over H3K9me3 in ERV silencing in endoderm cells. Our findings suggest, that H3K9me3 is not sufficient for ERV silencing, but rather critical for maintaining high DNA methylation.
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| Overall design |
RNA-seq of Embryo/ES/XEN/DE cells
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| Contributor(s) |
Wang Z, Fan R, Cernilogar F, Schotta G |
| Citation(s) |
36123357 |
| |
| Submission date |
Oct 20, 2019 |
| Last update date |
Oct 04, 2022 |
| Contact name |
Gunnar Schotta |
| E-mail(s) |
gunnar.schotta@bmc.med.lmu.de
|
| Phone |
089218075422
|
| Organization name |
LMU Munich
|
| Department |
Biomedical Center
|
| Street address |
Grosshaderner Strasse 9
|
| City |
Planegg-Martinsried |
| ZIP/Postal code |
82152 |
| Country |
Germany |
| |
|
| Platforms (1) |
| GPL18480 |
Illumina HiSeq 1500 (Mus musculus) |
|
| Samples (36)
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| This SubSeries is part of SuperSeries: |
| GSE139128 |
Dominant role of DNA methylation over H3K9me3 for IAP silencing in endoderm |
|
| Relations |
| BioProject |
PRJNA578553 |
| SRA |
SRP226379 |
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