|
| Status |
Public on Jan 06, 2020 |
| Title |
Translational profiling of microglia |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Several mouse lines have been developed to target microglia genetically; however, they lack specificity. Here, we use ribosome tagging and immunoprecipitation coupled to RNA-Seq in our newly developed mouse line to define the true microglial translatome.
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| |
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| Overall design |
Ribosome-mRNA complexes were isolated from whole brains from P2ry12-CreER; RPL22HA mice by immunoprecipitation. Transcript enrichment was evaluated by RNA-Seq of transcripts in the input and in the immunoprecipitated fractions.
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| |
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| Contributor(s) |
McKinsey G, Santander N, Arnold T |
| Citation(s) |
32573436 |
| |
| Submission date |
Oct 02, 2019 |
| Last update date |
Jul 27, 2020 |
| Contact name |
Thomas Arnold |
| E-mail(s) |
thomas.arnold@ucsf.edu
|
| Organization name |
University of California, San Francisco
|
| Street address |
4th street 1505
|
| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94158 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (6)
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|
| Relations |
| BioProject |
PRJNA575523 |
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