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| Status |
Public on Jul 31, 2020 |
| Title |
Observation of the Changes in Chemokine-related Gene Expression of Endometriotic Cyst Stromal Cells by Three-dimensional Collagen Gel Culture |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Endometriotic cyst stromal cells (ECSCs) were isolated from ovarian endometriotic cyst and cultured for 48 hours in the floating three-dimensional collagen gel culture or in the conventional two-dimensional culture. Total mRNAs were extracted and subjected to gene expression microarray.
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| Overall design |
ECSCs were isolated from ovarian endometriotic cyst and cultured for 48 hours in the floating three-dimensional collagen gel culture or in the conventional two-dimensional culture. Total mRNAs were extracted with an RNeasy Mini kit (Qiagen, Valencia, CA, USA). Then, the samples were subjected to a gene expression microarray analysis with a commercially available human mRNA microarray (SurePrint G3 Human GE Microarray Kit 8x60K v2).
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| Contributor(s) |
Zhu R |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Aug 27, 2019 |
| Last update date |
Aug 02, 2020 |
| Contact name |
Ruofei Zhu |
| E-mail(s) |
zrf1218@outlook.com
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| Organization name |
Oita University
|
| Street address |
hasamamachi,idaigaoka,1-1
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| City |
yufu |
| ZIP/Postal code |
8795593 |
| Country |
Japan |
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| Platforms (1) |
| GPL17077 |
Agilent-039494 SurePrint G3 Human GE v2 8x60K Microarray 039381 (Probe Name version) |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA562517 |
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