GEO Accession viewer

NCBI > GEO > Accession Display

Not logged in | Login

GEO help: Mouse over screen elements for information.

Series GSE1361

Query DataSets for GSE1361

Status

Public on May 04, 2004

Title

Concurrent Versus Individual Binding of HuR and AUF1 to Common Labile Target mRNAs

Organism

Homo sapiens

Experiment type

Expression profiling by array

Summary

RNA was isolated from material that had been immunoprecipitated (IP) from Hela cells using antibodies recognizing RNA-binding proteins HuR or AUF1, as well as using a control IgG1 antibody. RNA was reverse-transcribed in the presence of [alpha-33P]dCTP and the radiolabeled product used to hybridize human cDNA arrays. The experiment was repeated using three independent sample sets. The samples were numbered HuR-1, HuR-2, HuR-3, AUF1-1, AUF1-2, AUF1-3, IgG1-1, IgG1-2, IgG1-3. HuR represents RNA from IP reactions using an anti-HuR antibody, AUF1 represents RNA from IP reactions using an anti-AUF1 antibody and, IgG1 represents RNA from IP reactions using an anti-IgG1 antibody. The numbers 1, 2 and 3 correspond to the three independent experimental datasets.
Keywords = RNa-binding protein
Keywords = mRNA stability
Keywords = exosome
Keywords = polysome
Keywords = RNA motif
Keywords: ordered

Contributor(s)

Lal A, Mazan-Mamczarz K, Kawai T, Yang X, Martindale JL, Gorospe M

Citation(s)

15257295

Submission date

May 04, 2004

Last update date

Jan 17, 2013

Contact name

Myriam Gorospe

E-mail(s)

myriam-gorospe@nih.gov