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Status
Public on May 03, 2004
Title
NIA MGC, Mammalian Genome Collection
Technology type
spotted DNA/cDNA
Distribution
non-commercial
Organism
Homo sapiens Manufacturer
NIA Gene Expression and Genomics Unit
Manufacture protocol
PCR amplified cDNA of ~16,800 individual clones from the NIH Mammalian Genome Collection with sucrose cresol dye for visualization were denatured with NaOH and spotted onto S&S Nytran SuperCharge nylon membranes ~12cm X 8cm. Twenty-four 384 well plates are printed using a BioRobotics MicroGrid II Arrayer with a 384 pin head. Each of the 384 sub-arrays is a 5X5 grid of 24 spots and 1 blank.
Support
S&S Nytran SuperCharge Nylon
Description
The goal of the Mammalian Gene Collection (MGC), a trans-NIH initiative, is to provide full-length open reading frame (FL-ORF) clones for human, mouse, and rat genes. In 2005, the project added the cow cDNAs generated by Genome Canada. Initially, cDNA libraries provided the source of the clones. Recently, alternative methods based on gene-specific amplification have been developed to target the recovery of human and mouse genes absent from the MGC collection.
Web link
http://mgc.nci.nih.gov/ Contributor(s)
Becker KG , Woood WH Submission date
May 03, 2004
Last update date
Jun 22, 2020
Contact name
Supriyo De
Organization name
NIA-IRP, NIH
Department
Laboratory of Genetics and Genomics
Lab
Computational Biology & Genomics Core
Street address
251 Bayview Blvd
City
Baltimore
State/province
Maryland
ZIP/Postal code
21224
Country
USA
Samples (244)
GSM21962 , GSM21963 , GSM21964 , GSM21965 , GSM21966 , GSM21967
GSM21968 ,
GSM21969 ,
GSM21970 ,
GSM23923 ,
GSM23924 ,
GSM23925 ,
GSM23926 ,
GSM23927 ,
GSM23928 ,
GSM23929 ,
GSM23930 ,
GSM23931 ,
GSM23932 ,
GSM23933 ,
GSM23934 ,
GSM23951 ,
GSM23952 ,
GSM23953 ,
GSM23954 ,
GSM23955 ,
GSM23956 ,
GSM23957 ,
GSM23961 ,
GSM23962 ,
GSM23963 ,
GSM23964 ,
GSM23968 ,
GSM23972 ,
GSM23979 ,
GSM23980 ,
GSM23984 ,
GSM23985 ,
GSM23989 ,
GSM23991 ,
GSM23995 ,
GSM23997 ,
GSM23998 ,
GSM24003 ,
GSM24019 ,
GSM24020 ,
GSM24022 ,
GSM24023 ,
GSM24025 ,
GSM24026 ,
GSM24027 ,
GSM24044 ,
GSM24045 ,
GSM24046 ,
GSM24047 ,
GSM24048 ,
GSM24049 ,
GSM24050 ,
GSM24051 ,
GSM24052 ,
GSM24080 ,
GSM24081 ,
GSM24084 ,
GSM24085 ,
GSM24087 ,
GSM24088 ,
GSM24089 ,
GSM24090 ,
GSM24091 ,
GSM24092 ,
GSM24094 ,
GSM24095 ,
GSM24096 ,
GSM24097 ,
GSM24098 ,
GSM24099 ,
GSM24100 ,
GSM24101 ,
GSM24102 ,
GSM24103 ,
GSM24104 ,
GSM24105 ,
GSM24106 ,
GSM24107 ,
GSM24149 ,
GSM24150 ,
GSM24151 ,
GSM24152 ,
GSM24153 ,
GSM24154 ,
GSM24155 ,
GSM24156 ,
GSM24157 ,
GSM24158 ,
GSM24159 ,
GSM24160 ,
GSM24161 ,
GSM24162 ,
GSM24163 ,
GSM24164 ,
GSM24165 ,
GSM24166 ,
GSM24167 ,
GSM24168 ,
GSM24169 ,
GSM24170 ,
GSM24171 ,
GSM24172 ,
GSM24173 ,
GSM24174 ,
GSM24175 ,
GSM24176 ,
GSM24177 ,
GSM24178 ,
GSM24179 ,
GSM24180 ,
GSM24181 ,
GSM24182 ,
GSM24183 ,
GSM24184 ,
GSM24191 ,
GSM24204 ,
GSM24285 ,
GSM24286 ,
GSM24287 ,
GSM24288 ,
GSM24289 ,
GSM24290 ,
GSM24291 ,
GSM24292 ,
GSM24293 ,
GSM24294 ,
GSM24295 ,
GSM24296 ,
GSM24297 ,
GSM24298 ,
GSM24299 ,
GSM24300 ,
GSM24301 ,
GSM24302 ,
GSM24303 ,
GSM24304 ,
GSM24305 ,
GSM24306 ,
GSM24307 ,
GSM24308 ,
GSM24309 ,
GSM24310 ,
GSM24311 ,
GSM24312 ,
GSM24313 ,
GSM24314 ,
GSM24315 ,
GSM24316 ,
GSM24317 ,
GSM24318 ,
GSM24319 ,
GSM24320 ,
GSM24321 ,
GSM24322 ,
GSM24323 ,
GSM96451 ,
GSM96452 ,
GSM96453 ,
GSM96454 ,
GSM96455 ,
GSM96456 ,
GSM96457 ,
GSM96458 ,
GSM96459 ,
GSM96460 ,
GSM96461 ,
GSM96462 ,
GSM96463 ,
GSM96464 ,
GSM96465 ,
GSM96466 ,
GSM96467 ,
GSM96468 ,
GSM96469 ,
GSM96470 ,
GSM96471 ,
GSM96472 ,
GSM96473 ,
GSM96474 ,
GSM96475 ,
GSM96476 ,
GSM96477 ,
GSM96478 ,
GSM96479 ,
GSM96480 ,
GSM96481 ,
GSM96482 ,
GSM96483 ,
GSM140819 ,
GSM140820 ,
GSM140821 ,
GSM140822 ,
GSM140823 ,
GSM160896 ,
GSM160897 ,
GSM160898 ,
GSM160899 ,
GSM160900 ,
GSM160901 ,
GSM275674 ,
GSM275675 ,
GSM317356 ,
GSM317357 ,
GSM317358 ,
GSM317359 ,
GSM317360 ,
GSM317361 ,
GSM317362 ,
GSM317363 ,
GSM317364 ,
GSM1345957 ,
GSM1345958 ,
GSM1345959 ,
GSM1345960 ,
GSM1345961 ,
GSM1345962 ,
GSM1345963 ,
GSM1345964 ,
GSM1345965 ,
GSM1345966 ,
GSM1345967 ,
GSM1345968 ,
GSM1345969 ,
GSM1345970 ,
GSM1345971 ,
GSM1345972 ,
GSM1345973 ,
GSM1345974 ,
GSM1345975 ,
GSM1345976 ,
GSM1345977 ,
GSM1345978 ,
GSM1345979 ,
GSM1345980 ,
GSM1345981 ,
GSM1345982 ,
GSM1345983 ,
GSM1345984 Series (12)
GSE1361 Concurrent Versus Individual Binding of HuR and AUF1 to Common Labile Target mRNAs
GSE1433 TIAR represses c-Myc biosynthesis following ultraviolet light irradiation
GSE1440 TIAR represses c-Myc biosynthesis following ultraviolet light irradiation-2
GSE4226 Alzheimer Disease peripheral blood mononuclear cell expression
GSE4227 GSTM3 single nucleotide polymorphism (rs7483) associated with peripheral BMC expression in Alzheimer disease
GSE4229 Alzheimer disease diagnostic prediction using peripheral BMC gene expression
GSE6983 TRF2 Inhibition-Mediated Degradation Derepresses the Neuronal Differentiation Program
GSE12647 Gene expression profiling in human fibroblast after low-LET irradiation
GSE55777 Hippocampal neurons, nylon-1 experiments
GSE55778 Hippocampal neurons, nylon-2 experiments
GSE55779 DRG axons and cell bodies experiments
GSE55781 Systematic identification of 3'UTR regulatory elements in activity-dependent mRNA stability in hippocampal neurons
Data table header descriptions ID GB_ACC GeneBank accession number
CLONE_ID Image Clone ID
GENE_SYMBOL Gene symbol
GENE_NAME Descriptive gene name
SPOT_ID Indicator of blank positions on array
Data table ID GB_ACC CLONE_ID GENE_SYMBOL GENE_NAME SPOT_ID 1
BC003354 IMAGE:2899899
CALM2
calmodulin 2 (phosphorylase kinase, delta)
2
BC000873 IMAGE:3460570
GNB4
guanine nucleotide binding protein beta subunit 4
3
BC012304 IMAGE:3873814
KIAA0801
RNA helicase
4
BC008115 IMAGE:2900378
FLJ11269
hypothetical protein FLJ11269
5
BC012382 IMAGE:3872458
NA 4-14-03 242
6
BC001919 IMAGE:2819215
GTF2A2
general transcription factor IIA, 2, 12kDa
7
BC012812 IMAGE:2964582
FLJ12895
hypothetical protein FLJ12895
8
BC001423 IMAGE:3139753
PSME3
proteasome (prosome, macropain) activator subunit 3 (PA28 gamma; Ki)
9
BC001190 IMAGE:3354679
CKB
creatine kinase, brain
10
BC001177 IMAGE:3534272
U2AF1
U2(RNU2) small nuclear RNA auxillary factor 1
11
BC003644 IMAGE:3532812
CDK4
cyclin-dependent kinase 4
12
BC004958 IMAGE:3631907
LRRFIP1
leucine rich repeat (in FLII) interacting protein 1
13
BC008725 IMAGE:3162543
VAT1
vesicle amine transport protein 1 homolog (T californica)
14
BC006281 IMAGE:3958342
SH2D3A
SH2 domain containing 3A
15
BC007825 IMAGE:2989923
GALNS
galactosamine (N-acetyl)-6-sulfate sulfatase (Morquio syndrome, mucopolysaccharidosis type IVA)
16
BC008044 IMAGE:3614611
MGC16063
hypothetical protein MGC16063
17
BC005948 IMAGE:4246501
SMPX
small muscle protein, X-linked
18
BC009921 IMAGE:3506774
ZBRK1
zinc-finger protein ZBRK1
19
BC021222 IMAGE:4308662
CNNM2
cyclin M2
20
BC009271 IMAGE:3353595
UBE3A
ubiquitin protein ligase E3A (human papilloma virus E6-associated protein, Angelman syndrome)
9600 645 Kbytes .Supplementary data files not provided
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