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Series GSE134717 Query DataSets for GSE134717
Status Public on Oct 03, 2019
Title Non-transmissible measles virus vector with segmented RNA genome establishes different types of iPSCs from hematopoietic cells
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary We established iPSCs from CD34+ HPCs or CD3+ T cells by transducing measles virus vectors encording reprogramming genes. HPC-derived iPSCs and T-cell-derived iPSCs showed naive-like and primed pluripotent cell properties, respectively.
Overall design CD34+ and CD3+ cells were sorted from cord blood and transduced with measles virus vectors encoding OCT3/4, SOX2, KLF4 and L-MYC. The cells were cultured in regular human ESC culture media for 2-3 weeks. After development of colonies, the cells were harvested and dissociated, then cultured with in naive media or human ESC culture media. After several passages, total cell RNA was harvested and subjected to RNA seq.
Contributor(s) Takeda M, Tani K
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Submission date Jul 23, 2019
Last update date Oct 04, 2019
Contact name Kenzaburo Tani
Organization name The Institute of Medical Science, The University of Tokyo
Department Project Division of ALA Advanced Medical Research
Street address 4-6-1, Shirokanedai, Minato-ku
City Tokyo
ZIP/Postal code 108-8639
Country Japan
Platforms (2)
GPL18460 Illumina HiSeq 1500 (Homo sapiens)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (8)
GSM3964087 TMP-iPSC4-GFP Neg
GSM3964088 TMP-iPSC4-GFP Low
GSM3964089 TMP-iPSC4-GFP Hi
BioProject PRJNA556217
SRA SRP216034

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Supplementary file Size Download File type/resource
GSE134717_normalized_counts.txt.gz 964.1 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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