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| Status |
Public on Mar 30, 2020 |
| Title |
Genome-wide maps of HPSH-CTCF binding in prim-5 stage zebrafish embryos |
| Organism |
Danio rerio |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
CCCTC-binding factor (CTCF) is an architectural protein with context dependent functional outcomes. Although CTCF is conserved throughout Metazoan evolution, its functions have been only evaluated in a limited number of species. Here, we present the first analysis of CTCF binding in the zebrafish genome via chromatin immunoprecipitation followed by sequencing (ChIP-seq). To avoid the need of custom antibodies, we generated a transgenic line with an HA-tagged version of CTCF that enabled the identification of 37,625 CTCF peaks in 24 hours post-fertilization (hpf) embryos. We determined that CTCF peaks generally overlap with histone marks associated to enhancers and active promoters, and identified repeats that are enriched on CTCF binding sites. In addition, we show extended sites of CTCF binding as previously shown in mammals and lamprey, supporting the hypothesis of a CTCF “code” in vertebrates. Our results indicate a positive association between the abundance of CTCF at promoters and gene expression that could be explained by high DNA accessibility. Finally, analysis of chromosome conformation capture data confirms that histone marks associated with active chromatin are enriched at boundaries of topologically associating domains (TADs), but no general enrichment of CTCF peaks nor motifs is observed at these regions. Our zebrafish line and data generated will be important for future studies of genome organization aiming to characterize the roles of CTCF in non-mammalian vertebrates.
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| Overall design |
ChIP-seq screen for CTCF occupancy in 24 hpf zebrafish embryos, in duplicates
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| Contributor(s) |
Pérez-Rico YA, Barillot E, Shkumatava A |
| Citation(s) |
32334414 |
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| Submission date |
Jun 27, 2019 |
| Last update date |
May 07, 2020 |
| Contact name |
Yuvia Alheli Perez Rico |
| E-mail(s) |
yuvia.perez-rico@embl.de
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| Organization name |
Institut Curie
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| Street address |
26 rue d'Ulm
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| City |
Paris |
| ZIP/Postal code |
75005 |
| Country |
France |
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| Platforms (1) |
| GPL18413 |
Illumina HiSeq 2500 (Danio rerio) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA551454 |
| SRA |
SRP212205 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE133437_CTCF_24hpf_combined_reps_all_peaks.bed.gz |
604.5 Kb |
(ftp)(http) |
BED |
| GSE133437_CTCF_24hpf_combined_reps_signal_chrms.bw |
494.9 Mb |
(ftp)(http) |
BW |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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