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Series GSE130262 Query DataSets for GSE130262
Status Public on Dec 03, 2019
Title RNA-Seq in HEK293T human cells
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary RNA-Seq procedure was used for analysis of expression in HEK293T cells. Cells were separately lysed with Trizol reagent (Invotrogen). Total RNA was extracted from cell lysed with Trizol reagent and PureLink RNA Micro Kit (Invitrogen) according to manufacture instruction. RNA quality was checked with BioAnalyser and RNA 6000 Nano Kit (Agilent). Poly(A)+ RNA was purified with Dynabeads® mRNA Purification Kit (Ambion). Illumina library was prepared from poly(A)+RNA with NEBNextNEBNext® Ultra™II RNA Library Prep Kit for Illumina® (NEB) according to manual. Sequencing was performed on HiSeq1500 with 50 bp read length. At least 10 millions of reads were generated for each sample.
Overall design Analysis of HEK293T gene expression levels by RNA-Seq.
Contributor(s) Tchurikov NA, Kravatsky YV, Kretova OV
Citation(s) 31694324, 35328433, 35806206
Submission date Apr 24, 2019
Last update date Jul 15, 2022
Contact name Nickolai Tchurikov
Organization name Engelhardt Institute of Molecular Biology
Department Department of Epigenetic Mechanisms of Gene Expression Regulation
Street address 32, Vavilova str.
City Moscow
ZIP/Postal code 119991
Country Russia
Platforms (1)
GPL18460 Illumina HiSeq 1500 (Homo sapiens)
Samples (2)
GSM3734787 HEK293T RNA-Seq rep1
GSM3734788 HEK293T RNA-Seq rep2
BioProject PRJNA534479
SRA SRP193747

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE130262_RAW.tar 6.2 Mb (http)(custom) TAR (of TXT)
GSE130262_RNAseq.genes.results.txt.gz 3.1 Mb (ftp)(http) TXT 109.5 Mb (ftp)(http) BW 109.4 Mb (ftp)(http) BW
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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