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| Status |
Public on May 07, 2020 |
| Title |
Genetic ablation of maternal SoxB1 results in the delay of gastrulation gene regulatory program in zebrafish |
| Organism |
Danio rerio |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Purpose: Find out the consequences of Sox19b binding to the nucleosome distribution after ZGA
Methods: MNase-seq of WT and MZsox19b mutant zebrafish embryos at dome stage, building up nucleosome occupancy profiles
Results:Maternal Sox19b is involved in the nucleosome displacement from HNARs at the zygotic genome activation.
Conclusions: Nucleosome clearance by maternal Sox19b is essential for proper onset and timing of gastrulation.
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| Overall design |
Nucleosome occupancy profiles of dome staged wild type (WT) and MZsox19b zebrafish mutant were generated by MNase treatment followed by deep sequencing, using Illumina HiSeq 2500.
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| Contributor(s) |
Gao M, Veil M, Onichtchouk D |
| Citation(s) |
35145080 |
| |
| Submission date |
Jan 31, 2019 |
| Last update date |
Feb 06, 2023 |
| Contact name |
Daria Onichtchouk |
| E-mail(s) |
daria.onichtchouk@biologie.uni-freiburg.de
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| Phone |
00497612032595
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| Organization name |
University of Freiburg
|
| Street address |
Hauptstrasse 1
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| City |
Freiburg |
| ZIP/Postal code |
79104 |
| Country |
Germany |
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| Platforms (1) |
| GPL18413 |
Illumina HiSeq 2500 (Danio rerio) |
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| Samples (1) |
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| Relations |
| BioProject |
PRJNA518092 |
| SRA |
SRP183168 |
