|
| Status |
Public on Jan 22, 2019 |
| Title |
MicV, VrrA sRNA target identification |
| Organism |
Vibrio cholerae C6706 |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
In this study we determined the target spectra of the MicV and VrrA sRNAs via pulse-expression followed by RNA-sequencing.
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| Overall design |
mRNA profiles of V.cholerae ΔmicV ΔvrrA strains carrying pBAD-ctr (empty vector control), pBAD-micV or pBAD-vrrA (inducible sRNA expression vectors). The bacteria were grown to early stationary phase, expression of the sRNAs was induced by addition of L-arabinose, and RNA-samples were taken 10minutes after induction. Three indepent biological replicates for each condition are provided.
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| |
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| Contributor(s) |
Peschek N, Hoyos M, Herzog R, Förstner KU, Papenfort K |
| Citation(s) |
31313835 |
| |
| Submission date |
Jan 16, 2019 |
| Last update date |
Jul 18, 2019 |
| Contact name |
Kathrin Fröhlich |
| E-mail(s) |
kathrin.froehlich@uni-jena.de
|
| Organization name |
Friedrich-Schiller-Universität Jena
|
| Department |
Fakultät für Biowissenschaften, Bacterial RNA Biology
|
| Lab |
AG Fröhlich
|
| Street address |
Winzerlaer Straße 2
|
| City |
Jena |
| State/province |
Thuringia |
| ZIP/Postal code |
07745 |
| Country |
Germany |
| |
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| Platforms (1) |
| GPL26050 |
Illumina HiSeq 1500 (Vibrio cholerae C6706) |
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| Samples (9)
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| This SubSeries is part of SuperSeries: |
| GSE125224 |
A conserved seed-pairing domain affords small RNA-mediated stress resistance in enterobacteria |
|
| Relations |
| BioProject |
PRJNA515433 |
| SRA |
SRP179732 |
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