|
| Status |
Public on Oct 17, 2019 |
| Title |
Effector differentiation potential determined at the level of human CD8+ naïve T cell subsets [RNA-Seq] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Antigen recognition by CD8+ naïve T cells induces immune activation and rapid effector differentiation that ensures elimination of virally-infected and malignant cells. While signals from the extracellular milieu and downstream of the TCR have long been known to play a crucial role, it is currently unknown whether heterogeneity within the naïve CD8+ T cell pool shapes the potency of effector differentiation. We report the identification of two discrete subsets of human CD8+ TN cells, defined by positive and negative expression of the chemokine receptor CXCR3, with different potential to generate fully-differentiated effector T cells following antigen-specific stimulation. The CXCR3+ are more abundant than the CXCR3– naïve in peripheral blood and lymphoid tissues, retain enhanced expression of effector-related genes ex vivo and display features of the T cell receptor chain repertoire indicating enhanced capacity to bind the peptide:major histocompatibility complex. Likewise, a phenotypically equivalent murine CD8+ naïve population expressing high levels of CD5, a surrogate indicator of enhanced TCR sensitivity for self, preferentially generates short-lived effector cells following infection. Our findings imply that CD8+ T cell effector differentiation potential in humans is shaped by heterogeneity in the preimmune repertoire
|
| |
|
| Overall design |
18 samples
|
| |
|
| Contributor(s) |
De Simone G, Mazza EM, Tagliafico E, Tenedini E, Peano C, Ferrari F, Lugli E |
| Citation(s) |
31740485 |
| |
| Submission date |
Jan 15, 2019 |
| Last update date |
Dec 17, 2019 |
| Contact name |
Emilia Maria Cristina Mazza |
| E-mail(s) |
Emilia.Mazza@humanitasresearch.it
|
| Organization name |
Humanitas Clinical and Research Center
|
| Street address |
Via Alessandro Manzoni, 56
|
| City |
Rozzano, Milan |
| ZIP/Postal code |
20089 |
| Country |
Italy |
| |
|
| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
|
| Samples (18)
|
|
| This SubSeries is part of SuperSeries: |
| GSE125102 |
Effector differentiation potential determined at the level of human CD8+ naïve T cell subsets |
|
| Relations |
| BioProject |
PRJNA515251 |
| SRA |
SRP179502 |
Less...
More...