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| Status |
Public on Nov 01, 2019 |
| Title |
pNET-seq (Pol II Ser5) in Arabidopsis |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Other
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| Summary |
Intronic transposon elements (TEs) comprise a large proportion in eukaryotic genomes, but how they regulate the host genes remains to be explored. Our forward genetic screen disclosed the plant specific RNA polymerases IV and V in suppressing intronic TE-mediated cryptic transcription initiation of a chimeric transcripts at FLC (FLCTE). Initiation of FLCTE transcription is blocked by the locally formed intronic heterochromatin, and RNA Pol V direct associates and establishes intronic heterochromatin, thus inhibits the entry of RNA Pol II Ser5p and the occupancy of H3K4 methylation for promoting initiation. Genome-wide Pol II Ser5p native elongation transcription sequencing revealed that this is a common mechanism among intronic heterochromatin-containing genes. This study sheds light on deeply understanding the function of intronic heterochromatin on host genes expression in eukaryotic genome.
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| Overall design |
We conducted pNET-seq with Arabidopsis wild-type (Col-0) and nrpe1-11 mutant seedlings to proflie the Pol II (Ser5) associated RNA.
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| Contributor(s) |
Zhou J, Liu L, Sun Q |
| Citation(s) |
31647592 |
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| Submission date |
Jan 15, 2019 |
| Last update date |
Nov 01, 2019 |
| Contact name |
Jincong Zhou |
| E-mail(s) |
zhoujc14@tsinghua.org.cn
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| Organization name |
Tsinghua University
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| Street address |
Tsinghua Yuan
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| City |
Beijing |
| State/province |
- |
| ZIP/Postal code |
100084 |
| Country |
China |
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| Platforms (1) |
| GPL23157 |
HiSeq X Ten (Arabidopsis thaliana) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA515215 |
| SRA |
SRP179487 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE125096_RAW.tar |
244.5 Mb |
(http)(custom) |
TAR (of BW) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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