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| Status |
Public on Mar 01, 2019 |
| Title |
Transcriptomic analysis of primary human T cells and murine RAW 264.7 macrophages 3D cultured in different collagen densities |
| Organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The density of the extracellular matrix has a dramatic impact on the gene expression profile of T cells and macrophages. This may reflect impaired immune response of T cells and macrophages in solid tumors.
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| Overall design |
Quantification of RNA expression in human PBMCs enriched for T cells through the selection of non-adhenernt cells and a murine macrophage cell line (RAW 264.7) cultured in different densities of extracellular matrix (Type I collagen). T cells were isolated from PBMCs
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| |
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| Contributor(s) |
Madsen DH, Grøntved L |
| Citation(s) |
30867051 |
| |
| Submission date |
Jan 09, 2019 |
| Last update date |
Aug 08, 2019 |
| Contact name |
Lars Grøntved |
| E-mail(s) |
larsgr@bmb.sdu.dk
|
| Phone |
+45 24 60 14 06
|
| Organization name |
University of Southern Denmark
|
| Department |
Biochemistry and Molecular Biology
|
| Street address |
Campusvej 55
|
| City |
Odense |
| ZIP/Postal code |
5230 |
| Country |
Denmark |
| |
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| Platforms (2) |
| GPL18460 |
Illumina HiSeq 1500 (Homo sapiens) |
| GPL18480 |
Illumina HiSeq 1500 (Mus musculus) |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA514057 |
| SRA |
SRP178203 |
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