 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Dec 07, 2018 |
| Title |
Systematic transcriptome analysis of white tea based on microarray |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Emerging in vivo and vitro data suggest that white tea extract (WTE) is capable of favourably modulating metabolic syndrome, especially by ameliorating abnormal lipid metabolism. Microarray-based gene expression profiling was performed in HepG2 cells to analyze the effects of WTE from a systematic perspective. Gene Ontology and pathway analysis revealed that WTE significantly affected pathways related to lipid metabolism. WTE significantly downregulated apolipoprotein B (APOB) and microsomal triglyceride transfer protein (MTTP) expression and thereby reduced the production of very-low-density lipoprotein. In the meanwhile, WTE stimulated low-density lipoprotein-cholesterol (LDL-c) uptake through targeting low-density lipoprotein receptor (LDLR), as a consequence of the activation of sterol regulatory element-binding protein 2 (SREBP2) and peroxisome proliferator-activated receptor δ (PPARδ). Furthermore, WTE significantly downregulated triglycerides synthetic genes and reduced intracellular triglycerides accumulation. Besides, we demonstrated that the tea catechins epigallocatechin-3-gallate (EGCG) and epicatechin-3-gallate (ECG) are abundant in WTE and contribute to the regulation of cholesterol metabolism related genes, including LDLR, MTTP and APOB. Our findings suggest white tea plays important roles in ameliorating abnormal lipid metabolism in vitro.
|
| |
|
| Overall design |
HepG2 cells were treated with white tea extract (WTE) or DMSO (control group) for 24 hours. Gene expression of different groups were measured by the standard protocol for Agilent DNA chip. KEGG and Reactome analysis were performed to explore WTE functions on hepatic cells.
|
| |
|
| Contributor(s) |
Luo K, Xie L |
| Citation(s) |
32334376 |
| |
| Submission date |
Dec 06, 2018 |
| Last update date |
Dec 11, 2020 |
| Contact name |
kun luo |
| Organization name |
Tsinghua university
|
| Street address |
Haidian district
|
| City |
Beijing |
| State/province |
Beijing |
| ZIP/Postal code |
100084 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL17077 |
Agilent-039494 SurePrint G3 Human GE v2 8x60K Microarray 039381 (Probe Name version) |
|
| Samples (2) |
|
| Relations |
| BioProject |
PRJNA508632 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE123402_RAW.tar |
6.3 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
|
|
|
|
 |
