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Series GSE123250 Query DataSets for GSE123250
Status Public on Mar 11, 2021
Title Multi-omics Characterization of WNT Pathway Reactivation to ameliorate BET Inhibitor Resistance in Liver Cancer Cells
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary The goals of this study are to find drug response genes to different drug combinations in BET inhibitor resistant HepG2 cells. RSEM is taken for consequent data analysis, mapping each sample to the human genome (build hg19) to determine expressions of 57773 transcripts.
Overall design First, HepG2 and Hep3B cells were seeded into 15 cm dish in 2 replicates after treatments with a BET inhibitor (215 nM) or Control (vehicle DMSO) for 24h.

Then a series of mRNA profile in HepG2 cells is generated from different inhibitor combination treated samples during 72h by deep sequencing, in duplicate, using Illumina HiSeq 3000. NC represents control, treated with DMSO; 74 means treatments with BET inhibitor at 4000nM; 94 means treatments with CHIR98014 at 4000nM;749 means treatments with BET inhibitor at 4000nM and CHIR98014 at 72nM;794 means treatments with BET inhibitor at 25nM and CHIR98014 at 4000nM.
Web link
Contributor(s) Liu Y, Xue M
Citation(s) 33667649
Submission date Dec 03, 2018
Last update date Mar 12, 2021
Contact name Mengzhu Xue
Organization name Shanghai Advanced Research Institute
Street address No.100, Haike Road
City Shanghai
ZIP/Postal code 201210
Country China
Platforms (1)
GPL21290 Illumina HiSeq 3000 (Homo sapiens)
Samples (18)
GSM3498614 rep1_Hep3B_control
GSM3498615 rep2_Hep3B_control
GSM3498616 rep1_Hep3B_BETi
BioProject PRJNA507963
SRA SRP172043

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE123250_NC12_74rep12_94rep12_749rep12_794rep12TPM.genesymbol.txt.gz 904.7 Kb (ftp)(http) TXT
GSE123250_hep3b_hepg2allgroupTPM.txt.gz 849.1 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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