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| Status |
Public on Sep 30, 2008 |
| Title |
Brain_22A-Scrapie_End-point_PR04-39-to-46_using_GPL7118 |
| Organism |
Mus musculus |
| Experiment type |
Non-coding RNA profiling by array
|
| Summary |
Examining the transcriptional change in whole mouse (VM) brain miRNA expression during prion (22A) induced neurodegeneration in order to identify miRNAs that are differentially expressed. MicroRNAs (miRNAs) are small, non-coding RNA molecules which are emerging as key regulators of numerous cellular processes. Compelling evidence links miRNAs to the control of neuronal development and differentiation, however, little is known about their role in neurodegeneration. De-regulation of a unique subset of miRNAs may suggest a conserved, disease-specific pattern of differentially expressed miRNAs is associated with prion–induced neurodegeneration.
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| Overall design |
MiRNA extracted from the whole brains of 3 VM mice treated with mouse-adapted 22A scrapie strain were analyzed on GPL7118 (array was divided into top and bottom) for differential expression. Age-matched, PBS treated control mice were used for comparison (competitive hybridization). A technical replicate for each mouse was performed as well as a technical replicate for each of the dye-swaps.
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| Citation(s) |
18987751 |
| |
| Submission date |
Aug 03, 2008 |
| Last update date |
Mar 20, 2012 |
| Contact name |
Reuben Saba |
| E-mail(s) |
Reuben_Saba@phac-aspc.gc.ca
|
| Phone |
204-789-2000 (ext 3053)
|
| Fax |
204-789-5009
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| Organization name |
University of Manitoba: Public Health agency of Canada
|
| Department |
Medical Microbiology
|
| Lab |
Dr. Stephanie Booth Lab
|
| Street address |
1015 Arligton Street
|
| City |
Winnipeg |
| State/province |
Manitoba |
| ZIP/Postal code |
R3E 3R2 |
| Country |
Canada |
| |
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| Platforms (1) |
| GPL7118 |
PHAC_Booth_Lab_miRNA_microarray_v.2005 |
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| Samples (12)
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| GSM309558 |
Brain_22A-Scrapie_End-point_PR04-39_Cy3_Top |
| GSM309559 |
Brain_22A-Scrapie_End-point_PR04-39_Cy3_Bottom |
| GSM309618 |
Brain_22A-Scrapie_End-point_PR04-39_Cy5_Top |
| GSM309619 |
Brain_22A-Scrapie_End-point_PR04-39_Cy5_Bottom |
| GSM309620 |
Brain_22A-Scrapie_End-point_PR04-41_Cy3_Top |
| GSM309621 |
Brain_22A-Scrapie_End-point_PR04-41_Cy3_Bottom |
| GSM309622 |
Brain_22A-Scrapie_End-point_PR04-41_Cy5_Top |
| GSM309623 |
Brain_22A-Scrapie_End-point_PR04-41_Cy5_Bottom |
| GSM309624 |
Brain_22A-Scrapie_End-point_PR04-46_Cy3_Top |
| GSM309625 |
Brain_22A-Scrapie_End-point_PR04-46_Cy3_Bottom |
| GSM309626 |
Brain_22A-Scrapie_End-point_PR04-46_Cy5_Top |
| GSM309627 |
Brain_22A-Scrapie_End-point_PR04-46_Cy5_Bottom |
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| This SubSeries is part of SuperSeries: |
| GSE12354 |
Gene profiling of mouse brain infected with scrapie strain 22A_End-point analysis |
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| Relations |
| BioProject |
PRJNA113839 |
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