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Platform GPL7118 Query DataSets for GPL7118
Status Public on Sep 30, 2008
Title PHAC_Booth_Lab_miRNA_microarray_v.2005
Technology type spotted oligonucleotide
Distribution non-commercial
Organism Mus musculus
Manufacturer Public Health Agency of Canada microarray core facility
Manufacture protocol A microarray containing oligonucleotide probes complimentary to 557 miRNAs was constructed. These probes consisted of all the non-redundant miRNA sequences from the miRNA registry (Release 5.0). The arrays were spotted in-house on Corning® epoxide coated slides; Probes were spotted in duplicate and additionally two full arrays were printed per slide. The probes were in sense orientation relative to the mature miRNAs and thus complimentary to either the cDNA or aRNA that would be derived from the mature miRNAs. The probes also had a 15 nucleotide long poly(T) tract at the 5'end for steric distancing from the array surface. Each probe was printed in duplicate and the signal intensities for averaged.
 
 
Citation(s) 17164008
Submission date Aug 01, 2008
Last update date Sep 30, 2008
Contact name Reuben Saba
E-mail(s) Reuben_Saba@phac-aspc.gc.ca
Phone 204-789-2000 (ext 3053)
Fax 204-789-5009
Organization name University of Manitoba: Public Health agency of Canada
Department Medical Microbiology
Lab Dr. Stephanie Booth Lab
Street address 1015 Arligton Street
City Winnipeg
State/province Manitoba
ZIP/Postal code R3E 3R2
Country Canada
 
Samples (12) GSM309558, GSM309559, GSM309618, GSM309619, GSM309620, GSM309621 
Series (2)
GSE12321 Brain_22A-Scrapie_End-point_PR04-39-to-46_using_GPL7118
GSE12354 Gene profiling of mouse brain infected with scrapie strain 22A_End-point analysis

Data table header descriptions
ID
Spot Index Identity of each spot on the microarray
Grid Location of a miRNA on grid
Row Row location on grid
Column Cloumn location on grid
Sample ID Official name of miRNA
SEQUENCE miRNA probe sequence; oligonucleotide sequence of probe complimentary to cDNA derived from mature miRNA
miRNA_ID
SPOT_ID spot identifier

Data table
ID Spot Index Grid Row Column Sample ID SEQUENCE miRNA_ID SPOT_ID
1 1 1 1 1 osa-miR-396c TTTTTTTTTTTTTTTTTCCACAGCTTTCTTGAACTT osa-miR-396c
2 2 1 1 2 cel-miR-84 TTTTTTTTTTTTTTTTGAGGTAGTATGTAATATTGTA cel-miR-84
3 3 1 1 3 mmu-miR-131 TTTTTTTTTTTTTTTTAAAGCTAGATAACCGAAAGT mmu-miR-131
4 4 1 1 4 cel-miR-69 TTTTTTTTTTTTTTTTCGAAAATTAAAAAGTGTAGA cel-miR-69
5 5 1 1 5 dme-miR-100 TTTTTTTTTTTTTTTAACCCGTAAATCCGAACTTGTG dme-miR-100
6 6 1 1 6 miR-Lk-10 TTTTTTTTTTTTTTTCGGCCTTAGTCGTCGGGGTGATT miR-Lk-10
7 7 1 1 7 mmu-miR-344 TTTTTTTTTTTTTTTTGATCTAGCCAAAGCCTGACTGT mmu-miR-344
8 8 1 1 8 miR-Lk-23 TTTTTTTTTTTTTTTGTGCTAGGATTGGGGCT miR-Lk-23
9 9 1 1 9 mmu-let-7a-1 TTTTTTTTTTTTTTTTGAGGTAGTAGGTTGTATAGTT mmu-let-7a-1
10 10 1 1 10 mmu-miR-29b-1 TTTTTTTTTTTTTTTTAGCACCATTTGAAATCAGTGT mmu-miR-29b-1
11 11 1 1 11 mmu-miR-345 TTTTTTTTTTTTTTTTGCTGACCCCTAGTCCAGTGC mmu-miR-345
12 12 1 1 12 mmu-miR-29a TTTTTTTTTTTTTTTCTAGCACCATCTGAAATCGGTT mmu-miR-29a
13 13 1 2 1 mmu-miR-206 TTTTTTTTTTTTTTTTGGAATGTAAGGAAGTGTGTGG mmu-miR-206
14 14 1 2 2 dme-miR-219 TTTTTTTTTTTTTTTTGATTGTCCAAACGCAATTCTTG dme-miR-219
15 15 1 2 3 cel-lin-4 TTTTTTTTTTTTTTTTCCCTGAGACCTCAAGTGTGA cel-lin-4
16 16 1 2 4 dme-miR-5 TTTTTTTTTTTTTTTAAAGGAACGATCGTTGTGATATG dme-miR-5
17 17 1 2 5 osa-miR-396a TTTTTTTTTTTTTTTTTCCACAGCTTTCTTGAACTG osa-miR-396a
18 18 1 2 6 cel-miR-65 TTTTTTTTTTTTTTTTATGACACTGAAGCGTAACCGAA cel-miR-65
19 19 1 2 7 mmu-miR-190 TTTTTTTTTTTTTTTTGATATGTTTGATATATTAGGT mmu-miR-190
20 20 1 2 8 miR-302c TTTTTTTTTTTTTTTTAAGTGCTTCCATGTTTCAGTGG miR-302c

Total number of rows: 576

Table truncated, full table size 42 Kbytes.




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