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| Status |
Public on Dec 01, 2018 |
| Title |
Characterization of the genes that were regulated by feeding with CBM 588 |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
In the present study, we investigated the effect of CBM 588 on lifespan and multiple-stress resistance using Caenorhabditis elegans as a model animal. When adult C. elegans were fed a standard diet of Escherichia coli OP50 or CBM 588, the lifespan of the animals fed CBM 588 was significantly longer than that of animals fed OP50. Moreover, the worms fed CBM 588 were more resistant to certain stressors, including infections with pathogenic bacteria, UV irradiation, and the metal stressor Cu2+. CBM 588 failed to extend the lifespan of the daf-2/IR, daf-16/FOXO and skn-1/Nrf2 mutants. Transcriptional profiling comparing CBM 588-fed and control-fed animals suggested that DAF-16-dependent class II genes were regulated by CBM 588. In conclusion, CBM 588 extends the lifespan of C. elegans probably through regulation of the insulin/IGF-1 signaling (IIS) pathway and the Nrf2 transcription factor, and CBM 588 improves resistance to several stressors in C. elegans.
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| Overall design |
Transcriptional profiling of eight-day-old worms that were fed OP50 or CBM 588 for five days, by deep sequencing, using Illumina HiSeq.
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| Contributor(s) |
Kage-Nakadai E |
| Citation(s) |
30563064 |
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| Submission date |
Nov 30, 2018 |
| Last update date |
Mar 06, 2019 |
| Contact name |
Eriko Kage-Nakadai |
| E-mail(s) |
nakadai@omu.ac.jp
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| Organization name |
Osaka Metropolitan Univ.
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| Street address |
3-3-138, Sugimoto, Sumiyoshi-ku
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| City |
Osaka |
| State/province |
Osaka |
| ZIP/Postal code |
558-8585 |
| Country |
Japan |
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| Platforms (1) |
| GPL13657 |
Illumina HiSeq 2000 (Caenorhabditis elegans) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA507749 |
| SRA |
SRP171604 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE123163_RAW.tar |
3.7 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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