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| Status |
Public on Jun 03, 2009 |
| Title |
Caenorhabditis elegans as a Genomic Model for Toxicology |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Expression profiling by array
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| Summary |
Effective toxicological testing of the vast number of new and existing chemicals currently in use will require efficient and cost effective methods. We evaluated the utility of a simple, low cost toxicity testing system employing the nematode Caenorhabditis elegans to identify toxicologically relevant changes in gene expression.
Dichlorvos and fenamiphos, which are organophosphorous pesticides that inhibit acetylcholinesterase were chosen as model toxicants to test the usefulness of the C. elegans toxicity testing system, and mefloquine, which appears to perturb neuronal Ca++ homeostasis, provided an out-group for analysis.
Keywords: gene expression array-based (RNA / in situ oligonucleotide)
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| Overall design |
Concentrations of dichlorvos, fenamiphos, or mefloquine sufficient to prevent the development of 10%, 50%, or 90% of mid-vulval L4 worms to the early gravid adult stage at 24 h after exposure were determined in range finding experiments. For definitive experiments, mid-vulval L4 worms were exposed to one of the foregoing concentrations of dichlorvos, fenamiphos, or mefloquine for 8h. Exposures were performed in triplicate.
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| Contributor(s) |
Lewis JA, Szilagyi M, Gehman E, Dennis WE, Jackson DA |
| Citation(s) |
19402892 |
| |
| Submission date |
Jul 30, 2008 |
| Last update date |
Jul 06, 2016 |
| Contact name |
John A. Lewis |
| E-mail(s) |
john.a.lewis1@us.army.mil
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| URL |
http://usacehr.amedd.army.mil
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| Organization name |
U.S. Army Center for Environmental Health Research
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| Department |
Biomarkers Program
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| Street address |
568 Doughten Drive
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| City |
Ft. Detrick |
| State/province |
MD |
| ZIP/Postal code |
21702-5010 |
| Country |
USA |
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| Platforms (1) |
| GPL200 |
[Celegans] Affymetrix C. elegans Genome Array |
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| Samples (36)
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| Relations |
| BioProject |
PRJNA113595 |
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