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| Status |
Public on Sep 03, 2020 |
| Title |
Genome-wide miRNA and protein coding expression of Danaus plexippus fed on three different hosts. |
| Organism |
Danaus plexippus |
| Experiment type |
Expression profiling by high throughput sequencing
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
Second instar larvae of the monarch butterfly, Danaus plexippus, from a nonmigratory population in Irapuato, Mexico, were reared for twenty-four hours on three species of milkweed hosts: Asclepias curassavica, A. linaria, and Gomphocarpus physocarpus. The greatest differences in coding gene expression occurred in genes controlling growth and detoxification and were most extreme in comparisons between G. physocarpus and the two Asclepias. MicroRNAs are predicted to be involved as regulators of many of these processes, in particular miR-278 could be an important regulator of growth through Hippo signaling.
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| |
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| Overall design |
9 poly(A) and 9 sRNA libraries were prepared from Danaus pleixppus second instar larvae after feeding for 24 hours on three different hosts to identify genes potentially involved in host specificity.
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| |
|
| Contributor(s) |
Gonzalez-De-la-Rosa PM, Loustalot-Laclette MR, Abreu-Goodger C, Markow TA |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Sep 26, 2018 |
| Last update date |
Sep 03, 2020 |
| Contact name |
Pablo M. Gonzalez-De-la-Rosa |
| E-mail(s) |
pablo.gonzalez@cinvestav.mx
|
| Organization name |
Cinvestav
|
| Department |
Langebio
|
| Lab |
RNA computational genomics
|
| Street address |
Km. 9.6 Libramiento Norte Carr. Irapuato-León
|
| City |
irapuato |
| State/province |
guanajuato |
| ZIP/Postal code |
36821 |
| Country |
Mexico |
| |
|
| Platforms (1) |
| GPL25611 |
Illumina HiSeq 2500 (Danaus plexippus) |
|
| Samples (18)
|
| GSM3401793 |
RNA pooled from 5 larvae fed on Asclepias curassavica replicate 1 [AC-1] |
| GSM3401794 |
RNA pooled from 5 larvae fed on Asclepias curassavica replicate 2 [AC-2] |
| GSM3401795 |
RNA pooled from 5 larvae fed on Asclepias curassavica replicate 3 [AC-3] |
| GSM3401796 |
RNA pooled from 5 larvae fed on Asclepias linaria replicate 1 [AL-1] |
| GSM3401797 |
RNA pooled from 5 larvae fed on Asclepias linaria replicate 2 [AL-2] |
| GSM3401798 |
RNA pooled from 5 larvae fed on Asclepias linaria replicate 3 [AL-3] |
| GSM3401799 |
RNA pooled from 5 larvae fed on Gomphocarpus physocarpus replicate 1 [GP-1] |
| GSM3401800 |
RNA pooled from 5 larvae fed on Gomphocarpus physocarpus replicate 2 [GP-2] |
| GSM3401801 |
RNA pooled from 5 larvae fed on Gomphocarpus physocarpus replicate 3 [GP-3] |
| GSM3401802 |
RNA pooled from 5 larvae fed on Asclepias curassavica replicate 1 [sAC-1] |
| GSM3401803 |
RNA pooled from 5 larvae fed on Asclepias curassavica replicate 2 [sAC-2] |
| GSM3401804 |
RNA pooled from 5 larvae fed on Asclepias curassavica replicate 3 [sAC-3] |
| GSM3401805 |
RNA pooled from 5 larvae fed on Asclepias linaria replicate 1 [sAL-1] |
| GSM3401806 |
RNA pooled from 5 larvae fed on Asclepias linaria replicate 2 [sAL-2] |
| GSM3401807 |
RNA pooled from 5 larvae fed on Asclepias linaria replicate 3 [sAL-3] |
| GSM3401808 |
RNA pooled from 5 larvae fed on Gomphocarpus physocarpus replicate 1 [sGP-1] |
| GSM3401809 |
RNA pooled from 5 larvae fed on Gomphocarpus physocarpus replicate 2 [sGP-2] |
| GSM3401810 |
RNA pooled from 5 larvae fed on Gomphocarpus physocarpus replicate 3 [sGP-3] |
|
| Relations |
| BioProject |
PRJNA493222 |
| SRA |
SRP162652 |
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