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| Status |
Public on Jan 23, 2019 |
| Title |
Hif-1alpha deletion leads to adverse treatment efficacy in a mouse model of MLL-AF9 driven AML |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
This report explores the effect of Hif-1alpha deletion, using a conditional mouse model, together with standard chemotherapy regimen to evaluate leukemia stem cell (LSC) targeting in a murine model of MLL-AF9 driven acute myeloid leukemia (AML). This study reveals that Hif-1alpha-deleted leukemias displayed a faster disease progression after chemotherapy.
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| Overall design |
Single cell RNA-seq data were generated on the 10X emulsion platform (10X Genomics, Pleasanton, CA) according to the manufactures instructions. The single-cell RNAseq from the Chromium platform was processed using CellRanger v1.3 using default settings, and subsequent normalization, QC, filtering, and differential gene expression analysis was performed in R using Seurat v1.4 (http://www.satijalab.org/seurat). Dimension reduction was performed using DDRTree over three components.
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| Contributor(s) |
Velasco-Hernandez T, Soneji S, Hidalgo I, Erlandsson E, Cammenga J, Bryder D |
| Citation(s) |
30595549 |
| |
| Submission date |
Sep 05, 2018 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Shamit Soneji |
| Organization name |
BMC
|
| Street address |
Solvegatan 19
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| City |
Lund |
| ZIP/Postal code |
22184 |
| Country |
Sweden |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA489376 |
| SRA |
SRP159634 |
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