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| Status |
Public on Aug 06, 2019 |
| Title |
Bisulfite (methylome) sequencing of msh1 memory line and non-memory (normal looking) sibling plants with isogenic Col-0 wild-type control in Arabidopsis |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Methylation profiling by high throughput sequencing
|
| Summary |
Col-0 plants were transformed with MSH1 RNAi construct. Transgene positive plant was self pollinated and transgene was segregated in subsequent generation, and screened for presence of transgene using PCR assay. Of transgene null plants, 20% plants displayed delayed in flowering, smaller in size, and lighter green termed as memory phenotype. While rest transgene null plants displayed normal phenotype termed as non-memory.
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| |
|
| Overall design |
Five memory plants, four non-memory plants, and four Col-0 wild-type plants were sequenced.
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| |
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| Contributor(s) |
Yang X, Mackenzie S |
| Citation(s) |
32371941, 37264042 |
| Submission date |
Aug 21, 2018 |
| Last update date |
Aug 25, 2023 |
| Contact name |
Hardik Kundariya |
| E-mail(s) |
hsk13@psu.edu
|
| Organization name |
The Pennsylvania State University
|
| Street address |
361 N. Frear
|
| City |
University Park |
| State/province |
PA |
| ZIP/Postal code |
16801 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL23157 |
HiSeq X Ten (Arabidopsis thaliana) |
|
| Samples (13)
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|
| Relations |
| BioProject |
PRJNA487079 |
| SRA |
SRP158529 |
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