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| Status |
Public on Aug 15, 2019 |
| Title |
BCL6 confers KRAS-mutant NSCLCs resistance to BET inhibitors |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We report that OTX015 upregulates BCL6 in KRAS-mutant NSCLC cells. Considering that both BCL6 and BET proteins regulate target genes by affecting transcription, we performed an RNA-seq experiment to identify downstream genes which response to OTX015. Retinal mRNA profiles of A549 cells treated with DMSO (0.1% for 6 hr) and OTX015 (1 μM for 6 hr) were generated by deep sequencing, in triplicate, using the SOLiD v4 sequencing platform. The sequence reads that passed quality filters were analyzed at the transcript isoform level with two methods: Burrows-Wheeler Aligner (BWA) followed by ANOVA (ANOVA) and TopHat followed by Cufflinks. qRT-PCR validation was performed using TaqMan and SYBR Green assays.
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| Overall design |
Retinal mRNA profiles of A549 treated with 1μM OTX015 for 6 hr or not were generated by deep sequencing, in triplicate, using the Illumina HiSeq 2500 sequencing platform.
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| Citation missing |
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| |
| Submission date |
Aug 16, 2018 |
| Last update date |
Aug 17, 2019 |
| Contact name |
Lingyun Zheng |
| E-mail(s) |
zhenglingyun@gdpu.edu.cn
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| Organization name |
Guangdong Pharmaceutical University
|
| Department |
School of Life Sciences and Biopharmaceutics
|
| Street address |
280 Wai Huan Dong Lu, Guangzhou Higher Education Mega Center
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| City |
Guangzhou |
| State/province |
Guangdong |
| ZIP/Postal code |
510006 |
| Country |
China |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA486332 |
| SRA |
SRP158126 |
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